Stability and selectivity of unnatural DNA with five-membered-ring nucleobase analogues

被引：41

作者：

Berger, M ^{[1
]}

Luzzi, SD ^{[1
]}

Henry, AA ^{[1
]}

Romesberg, FE ^{[1
]}

机构：

[1] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2002年 / 124卷 / 07期

关键词：

D O I：

10.1021/ja012090t

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In an effort to develop an orthogonal third base pair for the storage of genetic information, thiophene and furan heterocycles have been examined as nucleobase analogues. The stability of the unnatural bases was evaluated in duplex DNA paired opposite other unnatural bases as well as opposite the natural bases. Several unnatural base pairs are identified that are both reasonably stable and strongly selective against mispairing with native bases. These results expand the potential nucleobase analogues with which the genetic alphabet may be expanded to include five-membered-ring heterocycles.

引用

页码：1222 / 1226

页数：5

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