Electron microscopy localization and characterization of functionalized composite organic-inorganic SERS nanoparticles on leukemia cells

被引：43

作者：

Koh, Ai Leen ^{[1
,2
,3
]}

Shachaf, Catherine M. ^{[4
]}

Elchuri, Sailaja ^{[4
]}

Nolan, Garry P. ^{[4
]}

Sinclair, Robert ^{[1
,2
]}

机构：

[1] Stanford Univ, Dept Mat Sci & Engn, Stanford, CA 94305 USA

[2] Stanford Univ, Stanford Nanocharacterizat Lab, Stanford, CA 94305 USA

[3] Stanford Univ, Dept Mech Engn, Stanford, CA 94305 USA

[4] Stanford Univ, Dept Microbiol & Immunol, Baxter Lab Genet Pharmacol, Stanford, CA 94305 USA

来源：

ULTRAMICROSCOPY | 2008年 / 109卷 / 01期

关键词：

SERS nanoparticles on cells; Scanning electron microscopy; Transmission electron microscopy; Scanning Auger electron spectroscopy;

D O I：

10.1016/j.ultramic.2008.09.004

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

We demonstrate the use of electron microscopy as a powerful characterization tool to identify and locate antibody-conjugated composite organic-inorganic nanoparticle (COINs) surface enhanced Raman scattering (SERS) nanoparticles on cells. U937 leukemia cells labeled with antibody CD54-conjugated COINs were characterized in their native, hydrated state using wet scanning electron microscopy (SEM) and in their dehydrated state using high-resolution SEM. In both cases, the backscattered electron (BSE) detector was used to detect and identify the silver constituents in COINs due to its high sensitivity to atomic number variations within a specimen. The imaging and analytical capabilities in the SEM were further complemented by higher resolution transmission electron microscopy (TEM) images and scanning Auger electron spectroscopy (AES) data to give reliable and high-resolution information about nanoparticles and their binding to cell surface antigens. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：111 / 121

页数：11

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