Inactivation of the phloem-specific dof zinc finger gene DAG1 affects response to light and integrity of the testa of Arabidopsis seeds

被引:85
作者
Papi, M
Sabatini, S
Altamura, MM
Hennig, L
Schäfer, E
Costantino, P
Vittorioso, P
机构
[1] Univ Roma La Sapienza, Ist Pasteur Fondaz Cenci Bolognetti, Dipartimento Genet & Biol Mol, I-00185 Rome, Italy
[2] Univ Roma La Sapienza, Dipartimento Biol Vegetale, I-00185 Rome, Italy
[3] Univ Freiburg, Inst Biol 2, D-79104 Freiburg, Germany
关键词
D O I
10.1104/pp.010488
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
We show here that seeds from the knockout mutant of the Arabidopsis DAG1 gene encoding a Dof zinc finger transcription factor have an altered response to red and far-red light. Mutant dag1 seeds are induced to germinate by much lower red light fluence rates, and germination reaches more quickly a point where it is independent of phytochrome signaling. Moreover, although microscopic analysis reveals no obvious structural alterations in the seed coat (testa) of dag1 seeds, staining assays with different dyes point to an abnormal fragility of the testa. By extensive in situ mRNA hybridization analysis we show here that the gene, which is not expressed in the embryo, is specifically expressed in the phloem of all organs of the mother plant.
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页码:411 / 417
页数:7
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