Antigen-antibody interactions in capillary electrophoresis

被引:51
作者
Heegaard, NHH
Kennedy, RT
机构
[1] State Serum Inst, Dept Autoimmunol, D-2300 Copenhagen S, Denmark
[2] Univ Florida, Dept Chem, Gainesville, FL 32611 USA
来源
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2002年 / 768卷 / 01期
关键词
reviews; antigens; antibodies;
D O I
10.1016/S0378-4347(01)00489-3
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Immunoreactions in combination with separations by capillary electrophoresis (CE) are increasingly being used to quantitate specific analytes in biological fluids. Both competitive and non-competitive approaches have been used for the purpose and. in selected cases, now compare favorably with conventional quantitative immunoassays with respect to concentration limits of detection. CE is also a useful method to evaluate antigen-antibody binding on-line and offers unique possibilities for binding constant estimates, also for weakly binding antibodies and antibody fragments. In this review we cover recent developments in the use of antigen-antibody interactions in conjunction with CE and conclude that continued development of miniaturization, on-line preconcentration and more sensitive detection schemes will contribute to the further dissemination of CE-based immunoassays building on already established affinity CE approaches. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:93 / 103
页数:11
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