Rapid identification and high sensitive detection of cancer cells on the gold nanoparticle interface by combined contact angle and electrochemical measurements

被引：37

作者：

He, Fang ^{[1
]}

Shen, Qin ^{[1
]}

Jiang, Hui ^{[1
]}

Zhou, Jian ^{[1
]}

Cheng, Jian ^{[2
]}

Guo, Dadong ^{[1
]}

Li, Qingning ^{[1
]}

Wang, Xuemei ^{[1
]}

Fu, Degang ^{[1
]}

Chen, Baoan ^{[2
]}

机构：

[1] Southeast Univ, Sch Biol Sci & Med Engn, Chien Shiung Wu Lab, State Key Lab Bioelect, Nanjing 210096, Peoples R China

[2] Southeast Univ, Affiliated Zhongda Hosp, Dept Hematol & Oncol, Nanjing 210009, Peoples R China

来源：

TALANTA | 2009年 / 77卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Gold nanoparticles; Cancer cell identification; Electrochemical detection; Contact angle measurement; QUARTZ-CRYSTAL MICROBALANCE; MULTIDRUG-RESISTANCE; IMMUNOSENSOR; IMMUNOASSAY; PATHOGENS;

D O I：

10.1016/j.talanta.2008.07.063

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In this study, we have proposed a novel strategy for the rapid identification and high sensitive detection of different kinds of cancer cells by means of electrochemical and contact angle measurements. A simple, unlabeled method based on the functionalized Au nanoparticles (GNPs) modified interface has been utilized to distinguish the different cancer cells, including lung cancer cells, liver cancer cells, drug sensitive leukemia K562/B.W cells and drug resistant leukemia K562/ADM cells. The relevant results indicate that under optimal conditions, this method can provide the quantitative determination of cancer cells. with a detection limit of similar to 10(3) cells mL(-1). Our observations demonstrate that the difference in the hydrophilic properties for target cellular surfaces and in the uptake efficiency of the anticancer drug daunorubicin for different cancer cells could be readily chosen as the elements of cancer identification and sensitive detection. This raises the possibility to advance the promising clinic diagnosis and monitoring of tumors with the aim of successful chemotherapy of human cancers. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：1009 / 1014

页数：6

共 26 条

[1] An optical biosensor for rapid and label-free detection of cells [J].