Cloning and characterisation of the human adenosine A3 receptor gene

被引：27

作者：

Murrison, EM ^{[1
]}

Goodson, SJ ^{[1
]}

Edbrooke, MR ^{[1
]}

Harris, CA ^{[1
]}

机构：

[1] GLAXO WELLCOME MED RES CTR,DIV CELLULAR SCI,STEVENAGE SG1 2NY,HERTS,ENGLAND

来源：

FEBS LETTERS | 1996年 / 384卷 / 03期

关键词：

adenosine A3 receptor; upstream sequence; intron;

D O I：

10.1016/0014-5793(96)00324-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have cloned the gene for the human adenosine A3 receptor and report characterisation of its intron/exon structure and upstream untranslated region. The open reading frame is interrupted by a single intron of approximately 2.2 kb, within the coding sequence for the second cytoplasmic loop. Sequence analysis of the upstream region reveals no TATA box but the transcriptional start site has been mapped to a common nucleotide in three tissues by 5'-RACE and RT-PCR analysis. Northern blotting, 5'-RACE PCR acid analysis of upstream sequences, have provided no evidence for the occurrence of further introns in the upstream untranslated sequence or of transcriptional regulation by alternative splicing in this region.

引用

页码：243 / 246

页数：4

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