Large-scale isolation and crystallization of epothilone D from Myxococcus xanthus cultures

被引：26

作者：

Arslanian, RL ^{[1
]}

Parker, CD ^{[1
]}

Wang, PK ^{[1
]}

McIntire, JR ^{[1
]}

Lau, J ^{[1
]}

Starks, C ^{[1
]}

Licari, PJ ^{[1
]}

机构：

[1] Kosan Biosci Inc, Hayward, CA 94545 USA

来源：

JOURNAL OF NATURAL PRODUCTS | 2002年 / 65卷 / 04期

关键词：

D O I：

10.1021/np010438s

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The introduction of the epothilone polyketide synthase (PKS) into Myxococcus xanthus has enabled the heterologous production of epothilone D (1) on a large scale. To isolate this valuable product from the fermentation medium, an economical, scalable, and high-yielding purification process was developed. With the crystallization of I from a binary solvent system that consisted of ethanol and water, the product was recovered as white crystals with a final purity of greater than or equal to97% (w/w). This is the first reported crystallization of 1.

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页码：570 / 572

页数：3

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