Large-scale isolation and crystallization of epothilone D from Myxococcus xanthus cultures

被引:26
作者
Arslanian, RL [1 ]
Parker, CD [1 ]
Wang, PK [1 ]
McIntire, JR [1 ]
Lau, J [1 ]
Starks, C [1 ]
Licari, PJ [1 ]
机构
[1] Kosan Biosci Inc, Hayward, CA 94545 USA
来源
JOURNAL OF NATURAL PRODUCTS | 2002年 / 65卷 / 04期
关键词
D O I
10.1021/np010438s
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The introduction of the epothilone polyketide synthase (PKS) into Myxococcus xanthus has enabled the heterologous production of epothilone D (1) on a large scale. To isolate this valuable product from the fermentation medium, an economical, scalable, and high-yielding purification process was developed. With the crystallization of I from a binary solvent system that consisted of ethanol and water, the product was recovered as white crystals with a final purity of greater than or equal to97% (w/w). This is the first reported crystallization of 1.
引用
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页码:570 / 572
页数:3
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