Transcriptional regulation of fatty acid biosynthesis in Streptococcus pneumoniae

被引:87
作者
Lu, YJ [1 ]
Rock, CO [1 ]
机构
[1] St Jude Childrens Res Hosp, Dept Infect Dis, Memphis, TN 38105 USA
关键词
D O I
10.1111/j.1365-2958.2005.04951.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The transcriptional regulation of membrane fatty acid composition in the human pathogen Streptococcus pneumoniae is distinct from the systems utilized in the model organisms Escherichia coli and Bacillus subtilis. The genes encoding the components of type II fatty acid biosynthesis cluster at a single location within the S. pneumoniae genome, and the second gene in this cluster (SPR0376) encodes a transcription factor (FabT) that belongs to the MarR superfamily. Derivatives of S. pneumoniae strain D39 were constructed that lacked functional FabT. This strain had significantly elevated levels of saturated fatty acids and longer chain lengths than the control strain, was unable to grow at pH 5.5 and had increased sensitivity to detergent. Eliminating FabT function increased the expression levels of all of fab genes with the notable exception of fabM. FabT was purified and bound to the DNA palindrome located within the promoter regions of the fabT and fabK genes within the cluster. The analysis of cells with increased expression of individual genes leads to a model where the physical properties of the S. pneumoniae membrane is controlled primarily by the activity of FabK, the enoyl reductase, which diverts intermediates to saturated fatty acid formation, in contrast to E. coli where FabB, an elongation condensing enzyme, pulls the pathway in the direction of unsaturated acid synthesis.
引用
收藏
页码:551 / 566
页数:16
相关论文
共 66 条
[41]   A new mechanism for anaerobic unsaturated fatty acid formation in Streptococcus pneumoniae [J].
Marrakchi, H ;
Choi, KH ;
Rock, CO .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (47) :44809-44816
[42]   BINDING OF PURIFIED MULTIPLE ANTIBIOTIC-RESISTANCE REPRESSOR PROTEIN (MARR) TO MAR OPERATOR SEQUENCES [J].
MARTIN, RG ;
ROSNER, JL .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (12) :5456-5460
[43]   Phylogenetic footprinting of transcription factor binding sites in proteobacterial genomes [J].
McCue, LA ;
Thompson, W ;
Carmack, CS ;
Ryan, MP ;
Liu, JS ;
Derbyshire, V ;
Lawrence, CE .
NUCLEIC ACIDS RESEARCH, 2001, 29 (03) :774-782
[44]   Structure of the complex between the antibiotic cerulenin and its target, β-ketoacyl-acyl carrier protein synthase [J].
Moche, M ;
Schneider, G ;
Edwards, P ;
Dehesh, K ;
Lindqvist, Y .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (10) :6031-6034
[45]   Evidence that the essential response regulator YycF in Streptococcus pneumoniae, modulates expression of fatty acid biosynthesis genes and alters membrane composition [J].
Mohedano, ML ;
Overweg, K ;
de la Fuente, A ;
Reuter, M ;
Altabe, S ;
Mulholland, F ;
de Mendoza, D ;
López, P ;
Wells, JM .
JOURNAL OF BACTERIOLOGY, 2005, 187 (07) :2357-2367
[46]   Transcriptional regulation and signature patterns revealed by microarray analyses of Streptococcus pneumoniae R6 challenged with sublethal concentrations of translation inhibitors [J].
Ng, WL ;
Kazmierczak, KM ;
Robertson, GT ;
Gilmour, R ;
Winkler, ME .
JOURNAL OF BACTERIOLOGY, 2003, 185 (01) :359-370
[47]   Protein kinase C as a molecular machine for decoding calcium and diacylglycerol signals [J].
Oancea, E ;
Meyer, T .
CELL, 1998, 95 (03) :307-318
[48]   ANTIBIOTIC CERULENIN, A NOVEL TOOL FOR BIOCHEMISTRY AS AN INHIBITOR OF FATTY-ACID SYNTHESIS [J].
OMURA, S .
BACTERIOLOGICAL REVIEWS, 1976, 40 (03) :681-697
[49]  
OVERATH P, 1969, EUR J BIOCHEM, V7, P559
[50]   Large-scale identification of virulence genes from Streptococcus pneumoniae [J].
Polissi, A ;
Pontiggia, A ;
Feger, G ;
Altieri, M ;
Mottl, H ;
Ferrari, L ;
Simon, D .
INFECTION AND IMMUNITY, 1998, 66 (12) :5620-5629