Geniposide, from Gardenia jasminoides Ellis, inhibits the inflammatory response in the primary mouse macrophages and mouse models

被引：196

作者：

Fu, Yunhe ^{[1
]}

Liu, Bo ^{[1
]}

Liu, Jinhua ^{[2
]}

Liu, Zhicheng ^{[1
]}

Liang, Dejie ^{[1
]}

Li, Fengyang ^{[1
]}

Li, Depeng ^{[1
]}

Cao, Yongguo ^{[1
]}

Zhang, Xichen ^{[1
]}

Zhang, Naisheng ^{[1
]}

Yang, Zhengtao ^{[1
]}

机构：

[1] Jilin Univ, Coll Anim Sci & Vet Med, Dept Clin Vet Med, Changchun 130062, Jilin Province, Peoples R China

[2] Jilin Univ, Jilin Entry Exit Inspect & Quarantine Bur, Changchun 130062, Jilin Province, Peoples R China

来源：

INTERNATIONAL IMMUNOPHARMACOLOGY | 2012年 / 14卷 / 04期

基金：

高等学校博士学科点专项科研基金; 中国国家自然科学基金;

关键词：

Geniposide; LPS; Acute lung injury; Inflammatory; TLR; NITRIC-OXIDE SYNTHASE; ACUTE LUNG INJURY; KAPPA-B; ACTIVATION; NEUROTOXICITY; MICROGLIA; ELEVATION; CYTOKINES; SEVERITY; RELEASE;

D O I：

10.1016/j.intimp.2012.07.006

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

071005 [微生物学]; 100108 [医学免疫学];

摘要：

Geniposide, a main iridoid glucoside component of gardenia fruit, has been known to exhibit antibacterial, anti-inflammatory and other important therapeutic activities. The objective of this study was to investigate the protective effects of geniposide on inflammation in lipopolysaccharide (LPS) stimulated primary mouse macrophages in vitro and U'S induced lung injury model in vivo. The expression of pro-inflammatory cytokines was determined by enzyme-linked immunosorbent assay (ELISA). Nuclear factor-kappa B (NF-kappa B), inhibitory kappa B (I kappa B alpha) protein, p38, extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK) and Toll-like receptor 4 (TLR4) were determined by Western blot Further analysis was carried out in mTLR4 and mMD-2 co-transfected HEK293 cells. The results showed that geniposide markedly inhibited the LPS-induced TNF-alpha, IL-6 and IL-1 beta production both in vitro and in vivo. Geniposide blocked the phosphorylation of I kappa B alpha, p65, p38. ERK and JNK in LPS stimulated primary mouse macrophages. Furthermore, geniposide inhibited the expression of 11.124 in LPS stimulated primary mouse macrophages and inhibited the LPS-induced IL-8 production in HEK293-mll.R4/MD-2 cells. In vivo study, it was also observed that geniposide attenuated lung histopathologic changes in the mouse models. These results suggest that geniposide exerts an anti-inflammatory property by down-regulating the expression of TLR4 up-regulated by U'S. Geniposide is highly effective in inhibiting acute lung injury and may be a promising potential therapeutic reagent for acute lung injury treatment. (c) 2012 Elsevier B.V. All rights reserved.

引用

页码：792 / 798

页数：7

共 32 条

[21]

The pathogenesis of sepsis - Factors that modulate the response to gram-negative bacterial infection [J].