Over-expression of plant 14-3-3 proteins in tobacco: Enhancement of the plasmalemma K+ conductance of mesophyll cells

被引：42

作者：

Saalbach, G ^{[1
]}

Schwerdel, M ^{[1
]}

Natura, G ^{[1
]}

Buschmann, P ^{[1
]}

Christov, V ^{[1
]}

Dahse, I ^{[1
]}

机构：

[1] UNIV JENA,INST BIOCHEM & BIOPHYS,D-07743 JENA,GERMANY

来源：

FEBS LETTERS | 1997年 / 413卷 / 02期

关键词：

14-3-3; protein; Vicia faba cotyledon; tobacco transformation; covalent modification; ion channel activation; signal transduction;

D O I：

10.1016/S0014-5793(97)00865-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two cDNA clones encoding 14-3-3 homologous proteins were isolated from Vicia faba. Deduced amino acid sequences share different degrees of homology with other plant 14-3-3 proteins, Both clones, under the control of the CaMV 35S promoter, were transformed into tobacco plants, Immunoblotting showed three different forms of ca, 31, 34, and 37 kDa, indicating a covalent modification of the expressed 14-3-3 proteins, These forms were mainly present in the microsomal fraction, Patch-clamp studies of mesophyll protoplasts of the transformants revealed a strongly enhanced K+ conductance compared to the wild type, This indicates the involvement of 14-3-3 proteins in ion channel regulation, presumably by modulating kinase activities or binding the channel, (C) 1997 Federation of European Biochemical Societies.

引用

页码：294 / 298

页数：5

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