The inhibitor protein of phosphorylated nitrate reductase from spinach (Spinacia oleracea) leaves is a 14-3-3 protein

被引：148

作者：

Bachmann, M

Huber, JL

Liao, PC

Gage, DA

Huber, SC

机构：

[1] USDA ARS,RALEIGH,NC 27695

[2] MICHIGAN STATE UNIV,DEPT BIOCHEM,E LANSING,MI 48824

[3] N CAROLINA STATE UNIV,DEPT HORT,RALEIGH,NC 27695

[4] N CAROLINA STATE UNIV,DEPT CROP SCI & BOT SCH,RALEIGH,NC 27695

来源：

FEBS LETTERS | 1996年 / 387卷 / 2-3期

关键词：

nitrate reductase; inhibitor; 14-3-3; proteins; protein phosphorylation;

D O I：

10.1016/0014-5793(96)00478-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The inhibitor protein (IP) that inactivates spinach leaf NADH:nitrate reductase (NR) has been identified for the first time as a member of the eukaryotic 14-33 protein family based on three lines of evidence. First, the sequence of an eight amino acid tryptic peptide, obtained from immunopurified IF, matched that of a highly conserved region of the 14-3-3 proteins, Second, an authentic member of the 14-3-3 family, recombinant Arabidopsis GF14 omega, caused inactivation of phospho-NR in a magnesium-dependent manner identical to IF. Third, an anti-GP14 monoclonal antibody cross-reacted with IP and anti-IP monoclonal antibodies cross-reacted with GF14 omega.

引用

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页码：127 / 131

页数：5

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