Peptidase activities in human semen

被引:54
作者
Fernández, D
Valdivia, A
Irazusta, J
Ochoa, C
Casis, L
机构
[1] Univ Basque Country, Sch Med, Dept Physiol, Bilbao, Spain
[2] Euskalduna Clin, Lab Seminol & Clin Embryol, Bilbao 48080, Spain
关键词
aminopeptidase; neprilysin; enkephalin; semen; spermatozoa; prostasome;
D O I
10.1016/S0196-9781(01)00622-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Enkephalins are one of the opioids present in human semen and to date their function in this tissue remains unknown. The present work studies enkephalin-degrading enzyme activities, puromycin-sensitive alanyl aminopeptidase (AAP-S), puromycin-insensitive alanyl aminopeptidase N (Ap N) and neprilysin (NEP) in human seminal fractions. AAP-S activity was not detected in any fractions, whereas Ap N appeared in soluble and particulate sperm fractions in seminal fluid and in prostasome fraction. With regard to NEP activity, this was exclusively located in prostasome membranes. The high activity values observed in the prostasome fraction suggested that these peptidases and their substrates could be involved in seminal physiology. (C) 2002 Elsevier Science Inc. All rights reserved.
引用
收藏
页码:461 / 468
页数:8
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