LEUHISTIN, A NEW INHIBITOR OF AMINOPEPTIDASE-M, PRODUCED BY BACILLUS-LATEROSPORUS BMI156-14F1 .1. TAXONOMY, PRODUCTION, ISOLATION, PHYSICOCHEMICAL PROPERTIES AND BIOLOGICAL-ACTIVITIES

被引：36

作者：

AOYAGI, T ^{[1
]}

YOSHIDA, S ^{[1
]}

MATSUDA, N ^{[1
]}

IKEDA, T ^{[1
]}

HAMADA, M ^{[1
]}

TAKEUCHI, T ^{[1
]}

机构：

[1] INST MICROBIAL CHEM,3-14-23 KAMIOSAKI,SHINAGAWA KU,TOKYO 141,JAPAN

来源：

JOURNAL OF ANTIBIOTICS | 1991年 / 44卷 / 06期

关键词：

D O I：

10.7164/antibiotics.44.573

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Leuhistin has been isolated from the culture broth of Bacillus laterosporus BMI156-14F1 as part of a program designed to find microorganism-produced inhibitors of aminopeptidase M (AP-M). It was purified by use of column chromatography on Sepabeads SP206, Amberlite IRC-50, MCI gel CHP-20P and Sephadex G-10 and then isolated as colorless needles. Leuhistin inhibits AP-M strongly and it also inhibits AP-A and AP-B weakly. It is competitive with the substrate, and the inhibition constant (Ki) was 2.3 x 10(-7) M.

引用

页码：573 / 578

页数：6

共 10 条

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