Prognostic value of apoptosis and apoptosis-associated proteins in salivary gland adenoid cystic carcinoma

被引:30
作者
Jia, L
Esguerra, RL
Tang, XF
Yin, HF
Sakamoto, K
Okada, N
Takagi, M
机构
[1] Tokyo Med & Dent Univ, Grad Sch, Div Oral Hlth Sci, Dept Oral Restitut,Bunkyo Ku, Tokyo 1138549, Japan
[2] Tokyo Med & Dent Univ, Dent Hosp, Dept Diagnost Oral Pathol, Tokyo, Japan
[3] Jilin Univ, Sch Stomatol, Dept Oral Pathol, Changchun 130023, Peoples R China
[4] Peking Univ, Hosp 1, Dept Pathol, Beijing 100871, Peoples R China
关键词
apoptosis; bcl-2; cumulative survival; mdm2; p53; salivary gland adenoid cystic carcinoma;
D O I
10.1111/j.1440-1827.2004.01611.x
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
The purpose of the present study was to investigate the level of apoptosis and expressions of p53, mdm2 and bcl-2 proteins in salivary gland adenoid cystic carcinoma (ACC) to determine potential relationships among apoptosis, apoptosis-associated proteins and clinical cumulative survival. Thirty-nine formalin-fixed, paraffin-embedded cases, cribriform (17), tubular (13) and solid (9), were studied by immunohistochemistry. Apoptosis detection and analysis were determined by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick-end labeling (TUNEL). There was an inverse significance between the apoptotic index (AI) and bcl-2 expression (P = 0.018), whereas no correlation was found between the AI and either p53 or mdm2 expression (P = 0.416 and P = 0.456). Co-expression of p53 and mdm2 was found in 22 cases (P = 0.037). Patients with p53-positive tumors had a worse prognosis than those with p53-negative tumors (P = 0.014). Patients with a high AI had a better cumulative survival than patients with a low AI (P = 0.038). The present study suggests that p53 expression and AI can be useful as prognostic values; bcl-2 protein plays a role in the down-regulation of apoptosis and is also potentially useful as a prognostic parameter in salivary gland ACC.
引用
收藏
页码:217 / 223
页数:7
相关论文
共 35 条
[1]   Transcriptional activation by p53, but not induction of the p21 gene, is essential for oncogene-mediated apoptosis [J].
Attardi, LD ;
Lowe, SW ;
Brugarolas, J ;
Jacks, T .
EMBO JOURNAL, 1996, 15 (14) :3693-3701
[2]   Apoptosis [J].
Cummings, MC ;
Winterford, CM ;
Walker, NI .
AMERICAN JOURNAL OF SURGICAL PATHOLOGY, 1997, 21 (01) :88-101
[3]  
DARDICK I, 1987, PATHOL ANNU, V22, P1
[4]   Immunohistochemical Mdm2 expression in minor salivary gland tumours and its relationship to p53 gene status [J].
de Araújo, VC ;
Martins, MT ;
Leite, KRM ;
Gomez, RS ;
de Araújo, NS .
ORAL ONCOLOGY, 2000, 36 (01) :67-69
[5]   Apoptosis and cell-cycle regulatory proteins in colorectal carcinoma: relationship to tumour stage and patient survival [J].
Elkablawy, MA ;
Maxwell, P ;
Williamson, K ;
Anderson, N ;
Hamilton, PW .
JOURNAL OF PATHOLOGY, 2001, 194 (04) :436-443
[6]  
Fujita S, 1999, ORAL DIS, V5, P117
[7]   IDENTIFICATION OF PROGRAMMED CELL-DEATH INSITU VIA SPECIFIC LABELING OF NUCLEAR-DNA FRAGMENTATION [J].
GAVRIELI, Y ;
SHERMAN, Y ;
BENSASSON, SA .
JOURNAL OF CELL BIOLOGY, 1992, 119 (03) :493-501
[8]  
GIROD SC, 1995, ANTICANCER RES, V15, P1453
[9]  
Green DR, 1996, J CELL BIOCHEM, V60, P33, DOI 10.1002/(SICI)1097-4644(19960101)60:1<33::AID-JCB6>3.0.CO
[10]  
2-4