Immunohistochemical Mdm2 expression in minor salivary gland tumours and its relationship to p53 gene status

被引：6

作者：

de Araújo, VC

Martins, MT

Leite, KRM

Gomez, RS

de Araújo, NS

机构：

[1] Univ Sao Paulo, Fac Odontol, Disciplina Patol Oral, Sao Paulo, Brazil

[2] Sirio Libanes Hosp, Sao Paulo, Brazil

[3] Univ Fed Minas Gerais, Sch Dent, Dept Oral Pathol, Belo Horizonte, MG, Brazil

来源：

ORAL ONCOLOGY | 2000年 / 36卷 / 01期

关键词：

salivary gland tumours; p53; Mdm2;

D O I：

10.1016/S1368-8375(99)00054-8

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Mdm2 protein is a cellular regulator of p53 protein activity. Minor salivary gland tumours were investigated for immunohistochemical expression of Mdm2 protein and for p53 gene status. Formalin-fixed sections were submitted to monoclonal antibody anti-Mdm2 through use of the streptavidin-biotin method. Nuclear immunoreactivity was scored 1 (0-25% nuclei positive), 2 (26-50%), 3 (51-75%) and 4 (> 75%). The scores found were: PLGA = 1-4; ACC = 3 and 4; ACA = 2 and 4; PA = 3. Genomic DNA of p53 gene exons 5-8 was examined by polymerase chain reaction and no alterations were detected. The strong immunohistochemical Mdm2 expression may represent an alternative mechanism to the development of salivary gland tumours. (C) 2000 Elsevier Science Ltd. All rights reserved.

引用

页码：67 / 69

页数：3

共 20 条

[1]

ARAUJO VC, 1997, EUROPEAN J CANC B, V33, P5

[2]

BOCKLAGE T, 1995, ARCH PATHOL LAB MED, V119, P69

[3]

DEGUCHI H, 1993, ACTA PATHOL JAPON, V43, P413

[4] CLINICAL IMPLICATIONS OF THE P53 TUMOR-SUPPRESSOR GENE [J].

HARRIS, CC ;

HOLLSTEIN, M .

NEW ENGLAND JOURNAL OF MEDICINE, 1993, 329 (18) :1318-1327

[5] Mdm2 promotes the rapid degradation of p53 [J].

Haupt, Y ;

Maya, R ;

Kazaz, A ;

Oren, M .

NATURE, 1997, 387 (6630) :296-299

[6]

ISHII K, 1994, PATHOL INT, V44, P287

[7] The tumorigenic potential and cell growth characteristics of p53-deficient cells are equivalent in the presence or absence of Mdm2 [J].

Jones, SN ;

Sands, AT ;

Hancock, AR ;

Vogel, H ;

Donehower, LA ;

Linke, SP ;

Wahl, GM ;

Bradley, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (24) :14106-14111

[8] Expression and mutations of p53 in salivary gland tumours [J].

Karja, VJ ;

Syrjanen, KJ ;

Kurvinen, AK ;

Syrjanen, SM .

JOURNAL OF ORAL PATHOLOGY & MEDICINE, 1997, 26 (05) :217-223

[9] mdm-2 oncogene expression in non-Hodgkin's lymphomas [J].

Kawamata, N ;

Miller, C ;

Levy, V ;

Shintaku, IP ;

Koeffler, HP ;

Said, JW .

DIAGNOSTIC MOLECULAR PATHOLOGY, 1996, 5 (01) :33-38

[10] Regulation of p53 stability by Mdm2 [J].

Kubbutat, MHG ;

Jones, SN ;

Vousden, KH .

NATURE, 1997, 387 (6630) :299-303

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