Interaction between the left-handed Z-DNA and polyamine-2 - The crystal structure of the d(CG)(3) and spermidine complex

被引：21

作者：

Ohishi, H

Nakanishi, I

Inubushi, K

vanderMarel, G

vanBoom, JH

Rich, A

Wang, AHJ

Hakoshima, T

Tomita, K

机构：

[1] OSAKA UNIV,FAC PHARMACEUT SCI,SUITA,OSAKA 565,JAPAN

[2] LEIDEN UNIV,GORLAEUS LABS,NL-2300 RA LEIDEN,NETHERLANDS

[3] MIT,DEPT BIOL,CAMBRIDGE,MA 02139

[4] UNIV ILLINOIS,URBANA,IL 61801

来源：

FEBS LETTERS | 1996年 / 391卷 / 1-2期

关键词：

Z-DNA; polyamine; d(CG)(3); spermidine; crystal structure; molecular complex; molecular interaction;

D O I：

10.1016/0014-5793(96)00723-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This paper deals with the crystal structure of d(CG)(3)-spermidine complex, The DNA fragment, d(CG)(3), was crystallized with N-(2-amino-propyl)-1,4-diamino-butane, PA(34), spermidine, The results of its X-ray crystallographic analysis showed many intermolecular contacts between d(CG)(3) and spermidine, but the binding mode of spermidine to the d(CG)(3) molecule is different from that of the d(CG)(3) and N-(2-amino-ethyl)-1,4-diamino-butane [PA(24)] complex: a spermidine molecule bound to the d(CG)(3) and its symmetrically related neighboring d(CG)(3) molecules through the water molecules with hydrogen bonds, while one PA(24) molecule connected directly to one d(CG)(3) molecule, but not to its neighboring d(CG)(3) molecule, In the crystal, the d(CG)(3) molecule was the left-handed Z-form, and three magnesium cations and a sodium cation were observed around the d(CG)(3) moiety with different binding modes from the case of the d(CG)(3)-PA(24) complex.

引用

页码：153 / 156

页数：4

共 12 条

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