Amperometric nitric oxide biosensor based on the immobilization of hemoglobin on a nanometer-sized gold colloid modified Au electrode

被引：23

作者：

Gu, HY ^{[1
]}

Yu, AM ^{[1
]}

Yuan, SS ^{[1
]}

Chen, HY ^{[1
]}

机构：

[1] Nanjing Univ, Dept Chem, Lab Mesoscop Mat & Chem, State Key Lab Coordinat Chem, Nanjing 210093, Peoples R China

来源：

ANALYTICAL LETTERS | 2002年 / 35卷 / 04期

基金：

中国国家自然科学基金;

关键词：

nitric oxide; hemoglobin; reagentless biosensor; gold colloid;

D O I：

10.1081/AL-120003167

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel reagentless NO biosensor based on hemoglobin (Hb) immobilized on nanometer-sized gold colloid particles associated with a cysteamine monolayer on gold electrode surface was presented. The experimental optimization was studied in detail. The immobilized Hb displayed an excellent electrocatalytic response to NO. The electrocatalytic currents were proportional to the NO concentration from 1.2 x 10(-8) to 5.0 x 10(-6) M and the detection limit was as low as 4.0 x 10(-9) M (S/N = 3) with good stability. Arginine, ascorbate, nitrite, uric acid, epinephrine and cystine didn't interfere with the determination of NO. The Michaelis-Menten constant (K-m) was determined to be 0.19 mM. This resulting biosensor has been applied to the direct determination of trace NO in raw blood.

引用

页码：647 / 661

页数：15

共 43 条

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