A phosphate bound universal linker for DNA synthesis

被引：11

作者：

Lyttle, MH ^{[1
]}

Dick, DJ ^{[1
]}

Hudson, D ^{[1
]}

Cook, RM ^{[1
]}

机构：

[1] Biosearch Technol Inc, Novato, CA 94949 USA

来源：

NUCLEOSIDES & NUCLEOTIDES | 1999年 / 18卷 / 08期

关键词：

D O I：

10.1080/07328319908044845

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A uridine-based linker immobilized onto polystyrene beads at the 5' terminus via a phosphodiester group and then used as a universal DNA synthesis support gives post synthesis DNA cleavage in 8 hrs or less without alkali metal salts. DNA produced with the new support was analyzed by HPLC, MALDI mass spectroscopy and PAGE. Each analysis showed DNA of equivalent quality to that produced with standard CPG supports, without contaminating materials resulting from linker or support backbone decomposition.

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页码：1809 / 1824

页数：16

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