Previous studies using artificial barriers at selected depths have shown the importance of N supply from soil below 15 cm to rice (Oryza sativa L.). Subsoil N availability and its use by wetland rice, however, have not been evaluated under normal field conditions. We examined distribution and availability of 2 M KCl-extractable N in 0- to 15-, 15- to 30-, and 30- to 45-cm layers of a submerged Alfisol during three successive crop seasons, as influenced by the depth of tillage imposed at the outset of the experiment. Mineral N availability in the 0- to 45-cm profile and rice N uptake in the first season were significantly higher (20-25 kg ha(-1) higher mineral N and 16-18 kg ha(-1) higher crop N uptake) with tillage to a depth of 40 cm than to 15 or 25 cm, the increased N supply originating largely from the 15- to 30-cm layer. In two following seasons, tillage to 40 cm gave the highest mineral N in the subsoil layers, and increased rice N uptake by 12 to 14 kg ha(-1) compared with tillage to 15 cm. The beneficial effects of deep tillage were attributed to the elimination of mechanical impedence to root proliferation, and lower susceptibility of subsoil N to various loss mechanisms. Initial and final analyses of Kjeldahl N in the soil profile indicated no adverse effect of practicing 40-cm-deep tillage once in three seasons on soil N fertility. Further study is needed to determine optimum depth and frequency of primary tillage for different soil types to enhance N use efficiency.