Solid-phase UV spectrophotometric method for determination of ciprofloxacin

被引：76

作者：

Pascual-Reguera, MI ^{[1
]}

Parras, GP ^{[1
]}

Díaz, AM ^{[1
]}

机构：

[1] Univ Jaen, Fac Expt Sci, Dept Phys & Analyt Chem, E-23071 Jaen, Spain

来源：

MICROCHEMICAL JOURNAL | 2004年 / 77卷 / 01期

关键词：

solid phase spectrophotometry; ciprofloxacin; pharmaceutical preparations;

D O I：

10.1016/j.microc.2004.01.003

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A simple and inexpensive method for the determination of ciprofloxacin has been developed using solid-phase spectrophotometry. The intrinsic absorbance of ciprofloxacin fixed on a dextran-type cation-exchange resin, Sephadex SP C-25, was measured directly at 277 and 380 nm after packing the gel beads in a 1-mm cell. Using a sample volume of 10 ml, the calibration graph was linear over the range 0.05-0.3 mug ml(-1) with a R.S.D. of 1.11% (n=8). The sensitivity obtained is 40 times higher than that of the corresponding solution method. The method was applied to the determination of ciprofloxacin in pharmaceuticel preparations and was validated by standard addition. (C) 2004 Elsevier B.V All rights reserved.

引用

页码：79 / 84

页数：6

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