Kuzbanian-mediated cleavage of Drosophila Notch

被引：185

作者：

Lieber, T ^{[1
]}

Kidd, S ^{[1
]}

Young, MW ^{[1
]}

机构：

[1] Rockefeller Univ, Genet Lab, New York, NY 10021 USA

来源：

GENES & DEVELOPMENT | 2002年 / 16卷 / 02期

关键词：

Notch; Kuzbanian; processing; Drosophila;

D O I：

10.1101/gad.942302

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Loss of Kuzbanian, a member of the ADAM family of metalloproteases, produces neurogenic phenotypes in Drosophila. It has been suggested that this results from a requirement for kuzbanian-mediated cleavage of the Notch ligand Delta. Using transgenic Drosophila expressing transmembrane Notch proteins, we show that kuzbanian, independent of any role in Delta processing, is required for the cleavage of Notch. We show that Kuzbanian can physically associate with Notch and that removal of kuzbanian activity by RNA-mediated interference in Drosophila tissue culture cells eliminates processing of ligand-independent transmembrane Notch molecules. Our data suggest that in Drosophila, kuzbanian can mediate S2 cleavage of Notch.

引用

收藏

页码：209 / 221

页数：13

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