Viscometry and atomic force microscopy studies of the interactions of a dimeric cyanine dye with DNA

被引：28

作者：

Bordelon, JA ^{[1
]}

Feierabend, KJ ^{[1
]}

Siddiqui, SA ^{[1
]}

Wright, LL ^{[1
]}

Petty, JT ^{[1
]}

机构：

[1] Furman Univ, Dept Chem, Greenville, SC 29613 USA

来源：

JOURNAL OF PHYSICAL CHEMISTRY B | 2002年 / 106卷 / 18期

关键词：

D O I：

10.1021/jp014680j

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The structures of the noncovalent complexes of DNA with a thiazole orange dimer (TOTO) were investigated in solution and when adsorbed to a mica substrate, using viscometry and atomic force microscopy (AFM), respectively. From the viscosity measurements at low TOTO:base pair concentrations, the dye lengthens the DNA helix by two times the base pair spacing, consistent with bisintercalation. Higher concentrations of TOTO cause a significant decrease in the viscosity of the DNA solutions, as also observed for related bifunctional ligands. The conformational changes induced by the dimer are compared to those of the monomer. The contour lengths of the DNA-TOTO complexes adsorbed onto mica were directly measured by AFM. These lengths and the size of the binding site are inconsistent with the bisintercalation binding mode observed in solution, suggesting an alternative mode of binding on mica.

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页码：4838 / 4843

页数：6

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