BMP9-Induced Osteogenetic Differentiation and Bone Formation of Muscle-Derived Stem Cells

被引：21

作者：

Li Xiang ^{[1
]}

Chen Liang ^{[1
]}

Ke Zhen-Yong ^{[1
]}

Yin Liang-Jun ^{[1
]}

Deng Zhong-Liang ^{[1
]}

机构：

[1] Chongqing Med Univ, Affiliated Hosp 2, Dept Orthopaed Surg, Chongqing 400010, Peoples R China

来源：

JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY | 2012年

关键词：

SKELETAL-MUSCLE; MORPHOGENETIC PROTEINS; REGENERATION; THERAPY; BMPS; MICE;

D O I：

10.1155/2012/610952

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

Efficient osteogenetic differentiation and bone formation from muscle-derived stem cells (MDSCs) should have potential clinical applications in treating nonunion fracture healing or bone defects. Here, we investigate osteogenetic differentiation ability of MDSCs induced by bone morphogenetic protein 9 (BMP9) in vitro and bone formation ability in rabbit radius defects repairing model. Rabbit's MDSCs were extracted by type I collagenase and trypsin methods, and BMP9 was introduced into MDSCs by infection with recombinant adenovirus. Effects of BMP9-induced osteogenetic differentiation of MDSCs were identified with alkaline phosphatase (ALP) activity and expression of later marker. In stem-cell implantation assay, MDSCs have also shown valuable potential bone formation ability induced by BMP9 in rabbit radius defects repairing test. Taken together, our findings suggest that MDSCs are potentiated osteogenetic stem cells which can be induced by BMP9 to treat large segmental bone defects, nonunion fracture, and/or osteoporotic fracture.

引用

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