共 52 条
The RSC nucleosome-remodeling complex is required for Cohesin's association with chromosome arms
被引:147
作者:
Huang, J
[1
]
Hsu, JM
[1
]
Laurent, BC
[1
]
机构:
[1] Suny Downstate Med Ctr, Program Mol & Cellular Biol, Dept Microbiol & Immunol, Morse Inst Mol Biol & Genet, Brooklyn, NY 11203 USA
关键词:
D O I:
10.1016/S1097-2765(04)00103-0
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
The fidelity of chromosome segregation requires that the cohesin protein complex bind together newly replicated sister chromatids both at centromeres and at discrete sites along chromosome arms. Segregation of the yeast 2 mu plasmid also requires cohesin, which is recruited to the plasmid partitioning locus. Here we report that the RSC chromatin-remodeling complex regulates the differential association of cohesin with centromeres and chromosome arms. RSC cycles on and off chromosomal arm and plasmid cohesin binding sites in a cell cycle-regulated manner 15 min preceding Mcd1p, the central cohesin subunit. We show that in rsc mutants Mcd1 p fails to associate with chromosome arms but still binds to centromeres, and that consequently, the arm regions of mitotic sister chromosomes separate precociously while cohesion at centromeres is unaffected. Our data suggest a role for RSC in facilitating the loading of cohesin specifically onto chromosome arms, thereby ensuring sister chromatid cohesion and proper chromosome segregation.
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页码:739 / 750
页数:12
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