Selection and optimization of microbial hosts for biofuels production

被引：275

作者：

Fischer, Curt R. ^{[1
]}

Klein-Marcuschamer, Daniel ^{[1
]}

Stephanopoulos, Gregory ^{[1
]}

机构：

[1] MIT, Dept Chem Engn, Cambridge, MA 02139 USA

来源：

METABOLIC ENGINEERING | 2008年 / 10卷 / 06期

基金：

美国国家科学基金会;

关键词：

Biofuels; Ethanol; Butanol; Strain optimization; Stress tolerance; Host organism;

D O I：

10.1016/j.ymben.2008.06.009

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Currently, the predominant microbially produced biofuel is starch- or sugar-derived ethanol. However, ethanol is not an ideal fuel molecule, and lignocellulosic feedstocks are considerably more abundant than both starch and sugar. Thus, many improvements in both the feedstock and the fuel have been proposed. In this paper, we examine the prospects for bioproduction of four second-generation biofuels (n-butanol, 2-butanol, terpenoids, or higher lipids) from four feedstocks (sugars and starches, lignocellulosics, syngas, and atmospheric carbon dioxide). The principal obstacle to commercial production of these fuels is that microbial catalysts of robust yields, productivities, and titers have yet to be developed. Suitable microbial hosts for biofuel production must tolerate process stresses such as end-product toxicity and tolerance to fermentation inhibitors in order to achieve high yields and titers. We tested seven fast-growing host organisms for tolerance to production stresses, and discuss several metabolic engineering strategies for the improvement of biofuels production. (C) 2008 Published by Elsevier Inc.

引用

页码：295 / 304

页数：10

共 120 条

[11]

Aukrust T W, 1995, Methods Mol Biol, V47, P201

[12] The hyperfluidization of mammalian cell membranes acts as a signal to initiate the heat shock protein response [J].