An indirect competitive immunoassay for insulin autoantibodies based on surface plasmon resonance

被引：21

作者：

Carlsson, Jenny ^{[1
]}

Gullstrand, Camilla ^{[2
]}

Westermark, Gunilla T. ^{[3
]}

Ludvigsson, Johnny ^{[2
]}

Enander, Karin ^{[1
]}

Liedberg, Bo ^{[1
]}

机构：

[1] Linkoping Univ, Dept Phys Chem & Biol IFM, Div Sensor Sci & Mol Phys, SE-58183 Linkoping, Sweden

[2] Linkoping Univ, Fac Hlth Sci, Dept Clin & Expt Med IKE, Diabet Res Ctr,Div Pediat, SE-58185 Linkoping, Sweden

[3] Linkoping Univ, Fac Hlth Sci, Dept Clin & Expt Med IKE, Diabet Res Ctr,Div Cell Biol, SE-58185 Linkoping, Sweden

来源：

BIOSENSORS & BIOELECTRONICS | 2008年 / 24卷 / 04期

基金：

瑞典研究理事会;

关键词：

SPR; Type; 1; diabetes; Insulin autoantibodies; Indirect competitive immunoassay; RIA;

D O I：

10.1016/j.bios.2008.07.018

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

We have developed a sensitive and specific method based on surface plasmon resonance (SPR) for detection of insulin autoantibodies (IAA) in serum samples from individuals at high risk of developing type 1 diabetes (T1D). When measuring trace molecules in undiluted sera with label-free techniques like SPIR, non-specific adsorption of matrix proteins to the sensor surface is often a problem, since it causes a signal that masks the analyte response. The developed method is an indirect competitive immunoassay designed to overcome these problems. Today, IAA is mainly measured in radio immunoassays (RIAs), which are time consuming and require radioactively labeled antigen. With our SPR-based immunoassay the overall assay time is reduced by a factor of > 100 (4 days to 50 min), while sensitivity is maintained at a level comparable to that offered by RIA. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：876 / 881

页数：6

共 34 条

[1] Natural history of type 1 diabetes [J].