Modification of fatty acid composition in tomato (Lycopersicon esculentum) by expression of a borage Δ6-desaturase

被引：15

作者：

Cook, D

Grierson, D

Jones, C

Wallace, A

West, G

Tucker, G

机构：

[1] Univ Nottingham, Sch Biosci, Div Nutr Biochem, Loughborough LE12 5RD, Leics, England

[2] Univ Nottingham, Sch Biosci, Div Plant Sci, Loughborough LE12 5RD, Leics, England

来源：

MOLECULAR BIOTECHNOLOGY | 2002年 / 21卷 / 02期

关键词：

tomato; fatty acids; desaturase; nutritional quality; genetic modification;

D O I：

10.1385/MB:21:2:123

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The improvement of nutritional quality is one potential application for the genetic modification of plants. One possible target for such manipulation is the modification of fatty acid metabolism. In this work, expression of a borage Delta(6)-desaturase cDNA in tomato (Lycopersicon esculentum L.) has been shown to produce gamma-linolenic acid (GLA; 18:3 Delta(6,9,12)) and octadecatetraenoic acid (OTA; 18:4 Delta(6,9,12,15)) in transgenic leaf and fruit tissue. This genetic modification has also, unexpectedly, resulted in a reduction in the percentage of linoleic acid (LA 18:2 Delta(9,12)) and a concomitant increase in the percentage of alpha-linolenic acid (ALA; 18:3 Delta(9,12,15)) in fruit tissue. These changes in fatty acid composition are thought to be beneficial for human health.

引用

页码：123 / 128

页数：6

共 14 条

[11] ISOLATION OF A DELTA-6-DESATURASE GENE FROM THE CYANOBACTERIUM SYNECHOCYSTIS SP STRAIN PCC-6803 BY GAIN-OF-FUNCTION EXPRESSION IN ANABAENA SP STRAIN PCC-7120 [J].