A comprehensive structure-function analysis of Arabidopsis SNI1 defines essential regions and transcriptional repressor activity

被引：111

作者：

Mosher, Rebecca A. ^{[1
]}

Durrant, Wendy E. ^{[1
]}

Wang, Dong ^{[1
]}

Song, Junqi ^{[1
]}

Dong, Xinnian ^{[1
]}

机构：

[1] Duke Univ, Dept Biol, Dev Cell & Mol Biol Grp, Durham, NC 27708 USA

来源：

PLANT CELL | 2006年 / 18卷 / 07期

关键词：

D O I：

10.1105/tpc.105.039677

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The expression of systemic acquired resistance (SAR) in plants involves the upregulation of many Pathogenesis-Related ( PR) genes, which work in concert to confer resistance to a broad spectrum of pathogens. Because SAR is a costly process, SAR-associated transcription must be tightly regulated. Arabidopsis thaliana SNI1 (for Suppressor of NPR1, Inducible) is a negative regulator of SAR required to dampen the basal expression of PR genes. Whole genome transcriptional profiling showed that in the sni1 mutant, Nonexpresser of PR genes (NPR1)-dependent benzothiadiazole S-methylester-responsive genes were specifically derepressed. Interestingly, SNI1 also repressed transcription when expressed in yeast, suggesting that it functions as an active transcriptional repressor through a highly conserved mechanism. Chromatin immunoprecipitation indicated that histone modification may be involved in SNI1-mediated repression. Sequence comparison with orthologs in other plant species and a saturating NAAIRS-scanning mutagenesis of SNI1 identified regions in SNI1 that are required for its activity. The structural similarity of SNI1 to Armadillo repeat proteins implies that SNI1 may form a scaffold for interaction with proteins that modulate transcription.

引用

页码：1750 / 1765

页数：16

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