Ovarian dynamics, serum estradiol and progesterone concentrations during the interovulatory interval in goats

Ovarian changes determined by daily transrectal ultrasonic scanning, and its correlation with serum progesterone (P4) and estradiol (E2) concentrations were studied in seven cyclic Saanen goats. Estrous cycles were synchronized with 2 injections of a PGF(2 alpha), analogue 9 d apart. All follicles greater than or equal to 2 mm in diameter and CL were measured each day. One goat showed a longer interestrous interval, associated with development of a cystic-luteinized structure. The mean interovulatory interval for the other 6 goats was 20.8+/-0.4 d. The incidence of goats with 4, 3, and 2 follicular waves was 3, 1 and 2 respectively; follicular waves emerged on Days 0.5+/-0.6, 7.2+/-0.7, 10.7+/-0.5 and 13.7+/-0.8 for Wave I, 2, 3 and the Ovulatory wave, respectively. The largest follicle of Wave 2 was smaller (4.9+/-0.1 mm) than the largest follicles of Wave 3 (6.2+/-0.1 mm, P less than or equal to 0.01) and of the Ovulatory wave (7.0+/-0.5 mm; P less than or equal to 0.01), and tended to be smaller than the largest follicle of Wave 1 (6.3+/-0.6 mm; P less than or equal to 0.09). Interval between emergence of Wave 1 and Wave 2 was longer than interval between emergence of Wave 2 and Wave 3 (7.3+/-0.9 d vs 4.0+/-0.4 d; P less than or equal to 0.01), and between Wave 3 and the Ovulatory wave (3.8+/-1.1 d; P less than or equal to 0.05). Two days before ovulation, the diameter of the ovulatory follicle was larger (P less than or equal to 0.01) than the first subordinate follicle. Serum E2 concentrations increased from the day of ovulation (2.7+/-0.3 pg/mL) to Day 2 (7.6+/-0.9 pg/mL; P less than or equal to 0.01), associated with the early-mid growing phase of the largest follicle of Wave 1, and then decreased to basal levels on Day 5 (P less than or equal to 0.01) and peaked again (16.5+/-2.4 pg/mL) 2 d before ovulation. The CL were detected ultrasonically on Day 3 post ovulation and attained a mean maximum diameter of 13.5+/-0.8 mm between Days 8 and 14. The following characteristics were observed: 1) ovarian follicular development in goats is wave-like; 2) increased P4 concentrations may be promoting follicular wave turnover; 3) it is suggested that the presence of follicular dominance and the production of E2 are different among waves. While in Wave I and in the Ovulatory wave, follicular dominance is present and production of E2 is consistent, no changes in serum E2 concentrations were found in other stages of the interovulatory interval. In the intervening waves, no indicators of follicular dominance could be firmly documented. (C) 1999 by Elsevier Science Inc.