The chicken HPRT gene:: a counter selectable marker for the DT40 cell line

被引：35

作者：

Fukagawa, T

Hayward, N

Yang, JA

Azzalin, C

Griffin, D

Stewart, AF

Brown, W

机构：

[1] Univ Oxford, Dept Biochem, Oxford OX1 3QU, England

[2] Brunel Univ, Dept Biol & Biochem, Uxbridge UB8 3PH, Middx, England

[3] European Mol Biol Lab, D-69117 Heidelberg, Germany

来源：

NUCLEIC ACIDS RESEARCH | 1999年 / 27卷 / 09期

关键词：

D O I：

10.1093/nar/27.9.1966

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe the cloning, characterisation and chromosomal mapping of the chicken hprt gene together with the construction of two counter selectable hprt(-/-) DT40 derived cell lines; One of these cell lines contains a stably integrated gene encoding a conditionally active cre recombinase and thus allows efficient manipulation of targeted loci by site-specific recombination. These cell lines will enhance the utility of the hyper-recombinogenic DT40 cell line as a system for the genetic analysis of cell autonomous functions in vertebrates and as a tool for mammalian chromosome engineering.

引用

收藏

页码：1966 / 1969

页数：4

相关论文

共 19 条

[1] Inducible expression based on regulated recombination: a single vector strategy for stable expression in cultured cells [J].

Angrand, PO ;

Woodroofe, CP ;

Buchholz, F ;

Stewart, AF .

NUCLEIC ACIDS RESEARCH, 1998, 26 (13) :3263-3269

[2] Targeted integration of DNA using mutant lox sites in embryonic stem cells [J].

Araki, K ;

Araki, M ;

Yamamura, KI .

NUCLEIC ACIDS RESEARCH, 1997, 25 (04) :868-872

[3] INCREASED RATIO OF TARGETED TO RANDOM INTEGRATION AFTER TRANSFECTION OF CHICKEN B-CELL LINES [J].

BUERSTEDDE, JM ;

TAKEDA, S .

CELL, 1991, 67 (01) :179-188

[4] HPRT LOCUS [J].

CASKEY, CT ;

KRUH, GD .

CELL, 1979, 16 (01) :1-9

[5] LOCATION OF CROSSOVERS DURING GENE TARGETING WITH INSERTION AND REPLACEMENT VECTORS [J].

DENG, CX ;

THOMAS, KR ;

CAPECCHI, MR .

MOLECULAR AND CELLULAR BIOLOGY, 1993, 13 (04) :2134-2140

[6] Efficient modification of human chromosomal alleles using recombination-proficient chicken human microcell hybrids [J].

Dieken, ES ;

Epner, EM ;

Fiering, S ;

Fournier, REK ;

Groudine, M .

NATURE GENETICS, 1996, 12 (02) :174-182

[7] Efficient conditional mutation of the vertebrate CENP-C gene [J].

Fukagawa, T ;

Brown, WRA .

HUMAN MOLECULAR GENETICS, 1997, 6 (13) :2301-2308

[8] Continental breakup and the ordinal diversification of birds and mammals [J].

Hedges, SB ;

Parker, PH ;

Sibley, CG ;

Kumar, S .

NATURE, 1996, 381 (6579) :226-229

[9] THE ORGANIZATION OF THE HUMAN HPRT GENE [J].

KIM, SH ;

MOORES, JC ;

DAVID, D ;

RESPESS, JG ;

JOLLY, DJ ;

FRIEDMANN, T .

NUCLEIC ACIDS RESEARCH, 1986, 14 (07) :3103-3118

[10] Efficient modification of a human chromosome by telomere-directed truncation in high homologous recombination-proficient chicken DT40 cells [J].

Kuroiwa, Y ;

Shinohara, T ;

Notsu, T ;

Tomizuka, K ;

Yoshida, H ;

Takeda, S ;

Oshimura, M ;

Ishida, I .

NUCLEIC ACIDS RESEARCH, 1998, 26 (14) :3447-3448