Efficient modification of a human chromosome by telomere-directed truncation in high homologous recombination-proficient chicken DT40 cells

被引：47

作者：

Kuroiwa, Y

Shinohara, T

Notsu, T

Tomizuka, K

Yoshida, H

Takeda, S

Oshimura, M

Ishida, I

机构：

[1] Kirin Brewery Co Ltd, Cent Labs Key Technol, Kanazawa Ku, Yokohama, Kanagawa 2360004, Japan

[2] Tottori Univ, Fac Med, Sch Life Sci, Dept Mol & Cell Genet, Yonago, Tottori 6830826, Japan

[3] Kyoto Univ, Fac Med, Dept Mol Immunol, Kyoto 60601, Japan

[4] CREST JST, Yonago, Tottori 6830826, Japan

来源：

NUCLEIC ACIDS RESEARCH | 1998年 / 26卷 / 14期

关键词：

D O I：

10.1093/nar/26.14.3447

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Truncation of human chromosomes at desired sites by homologous recombination techniques enables functional and structural analyses of human chromosomes and development of human artificial chromosomes. However, this targeted truncation has been inefficient. We describe here an efficient method for targeted truncation in the chicken DT40 cells with a high homologous recombination rate. The human chromosome 22 was transferred into DT40 cells, where human telomeric repeat (TTAGGG)n was targeted to the LIF locus on the chromosome. Molecular and cytogenetic analyses showed that the predicted truncation at the LIF locus occurred in all of the targeted clones.

引用

页码：3447 / 3448

页数：2

共 8 条

[1] INCREASED RATIO OF TARGETED TO RANDOM INTEGRATION AFTER TRANSFECTION OF CHICKEN B-CELL LINES [J].