Recurrent chromosomal abnormalities in human embryonic stem cells

被引：182

作者：

Spits, Claudia ^{[1
]}

Mateizel, Ileana ^{[1
]}

Geens, Mieke ^{[1
]}

Mertzanidou, Afroditi ^{[1
]}

Staessen, Catherine ^{[2
]}

Vandeskelde, Yves ^{[2
]}

Van der Elst, Josiane ^{[1
,3
]}

Liebaers, Inge ^{[1
,2
]}

Sermon, Karen ^{[1
]}

机构：

[1] Vrije Univ Brussels, Dept Embryol & Genet, B-1090 Brussels, Belgium

[2] Vrije Univ Brussels, UZ Brussel, Ctr Med Genet, B-1090 Brussels, Belgium

[3] Vrije Univ Brussels, Ctr Reprod Med, UZ Brussel, B-1090 Brussels, Belgium

来源：

NATURE BIOTECHNOLOGY | 2008年 / 26卷 / 12期

关键词：

D O I：

10.1038/nbt.1510

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Cultured human embryonic stem (hES) cells have a known predisposition to aneuploidy of chromosomes 12, 17 and X. We studied 17 hES cell lines by array-based comparative genomic hybridization (aCGH) and found that the cells accumulate other recurrent chromosomal abnormalities, including amplification at 20q11.21 and a derivative chromosome 18. These genomic changes have a variable impact at the transcriptional level.

引用

页码：1361 / 1363

页数：3

共 13 条

[1] Adaptation to culture of human embryonic stem cells and oncogenesis in vivo [J].

Baker, Duncan E. C. ;

Harrison, Neil J. ;

Maltby, Edna ;

Smith, Kath ;

Moore, Harry D. ;

Shaw, Pamela J. ;

Heath, Paul R. ;

Holden, Hazel ;

Andrews, Peter W. .

NATURE BIOTECHNOLOGY, 2007, 25 (02) :207-215

[2] Chromosomal breakpoints in primary colon cancer cluster at sites of structural variants in the genome [J].

Camps, Jordi ;

Grade, Marian ;

Nguyen, Quang Tri ;

Hoermann, Patrick ;

Becker, Sandra ;

Hummon, Amanda B. ;

Rodriguez, Virginia ;

Chandrasekharappa, Settara ;

Chen, Yidong ;

Difilippantonio, Michael J. ;

Becker, Heinz ;

Ghadimi, B. Michael ;

Ried, Thomas .

CANCER RESEARCH, 2008, 68 (05) :1284-1295

[3] Array comparative genomic hybridization analysis of olfactory neuroblastoma [J].

Guled, Mohamed ;

Myllykangas, Samuel ;

Frierson, Henry F., Jr. ;

Mills, Stacey E. ;

Knuutila, Sakari ;

Stelow, Edward B. .

MODERN PATHOLOGY, 2008, 21 (06) :770-778

[4] CD30 is a survival factor and a biomarker for transformed human pluripotent stem cells [J].

Herszfeld, D ;

Wolvetang, E ;

Langton-Bunker, E ;

Chung, TL ;

Filipczyk, AA ;

Houssami, S ;

Jamshidi, P ;

Koh, K ;

Laslett, AL ;

Michalska, A ;

Nguyen, L ;

Reubinoff, BE ;

Tellis, I ;

Auerbach, JM ;

Ording, CJ ;

Looijenga, LHJ ;

Pera, MF .

NATURE BIOTECHNOLOGY, 2006, 24 (03) :351-357

[5] Comparative genomic hybridization and karyotyping of human embryonic stem cells reveals the occurrence of an isodicentric X chromosome after long-term cultivation [J].

Inzunza, J ;

Sahlén, S ;

Holmberg, K ;

Strömberg, AM ;

Teerijoki, H ;

Blennow, E ;

Hovatta, O ;

Malmgren, H .

MOLECULAR HUMAN REPRODUCTION, 2004, 10 (06) :461-466

[6] Molecular cytogenetics: making it safe for human embryonic stem cells to enter the clinic [J].

Josephson, Richard .

EXPERT REVIEW OF MOLECULAR DIAGNOSTICS, 2007, 7 (04) :395-406

[7] Human chromosome fragility [J].

Lukusa, T. ;

Fryns, J. P. .

BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS, 2008, 1779 (01) :3-16

[8] Genomic alterations in cultured human embryonic stem cells [J].

Maitra, A ;

Arking, DE ;

Shivapurkar, N ;

Ikeda, M ;

Stastny, V ;

Kassauei, K ;

Sui, GP ;

Cutler, DJ ;

Liu, Y ;

Brimble, SN ;

Noaksson, K ;

Hyllner, J ;

Schulz, TC ;

Zeng, XM ;

Freed, WJ ;

Crook, J ;

Abraham, S ;

Colman, A ;

Sartipy, P ;

Matsui, SI ;

Carpenter, M ;

Gazdar, AF ;

Rao, M ;

Chakravarti, A .

NATURE GENETICS, 2005, 37 (10) :1099-1103

[9] Derivation of human embryonic stem cell lines from embryos obtained after IVF and after PGD for monogenic disorders [J].

Mateizel, I ;

De Temmerman, N ;

Ullmann, U ;

Cauffman, G ;

Sermon, K ;

Van de Velde, H ;

De Rycke, M ;

Degreef, E ;

Devroey, P ;

Liebaers, I ;

Van Steirteghem, A .

HUMAN REPRODUCTION, 2006, 21 (02) :503-511

[10] Mechanisms of DNA double-strand break repair and their potential to induce chromosomal aberrations [J].

Pfeiffer, P ;

Goedecke, W ;

Obe, G .

MUTAGENESIS, 2000, 15 (04) :289-302

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