Measuring the effect of glutamate receptor agonists on extracellular D-serine concentrations in the rat striatum using online microdialysis-capillary electrophoresis

被引:24
作者
Ciriacks, CM [1 ]
Bowser, MT [1 ]
机构
[1] Univ Minnesota, Dept Chem, Minneapolis, MN 55455 USA
关键词
D-Serine; astrocyte; capillary electrophoresis; microdialysis; AMPA receptor; NMDA receptor;
D O I
10.1016/j.neulet.2005.09.080
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Online microdialysis-capillary electrophoresis (CE) was used to measure the changes in extracellular D-serine concentration in response to the application of glutamate agonists and antagonists in the rat striatum. The microdialysis-CE assay was capable of measuring concentration changes as small as 8% with a sampling rate of 12-15 s. Kainic acid (KA) induced increases in D- and L-serine concentrations. Application of the non-NMDA receptor antagonist CNQX did not affect the increases observed for D- or L-serine, suggesting a nonspecific effect. NMDA also induced increases in D-serine, L-serine, glutamate and GABA concentrations. These increases were attenuated by the NMDA receptor antagonist MK-801. (c) 2005 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:200 / 205
页数:6
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