Differential identification of Mycobacterium tuberculosis complex and nontuberculous mycobacteria by duplex PCR assay using the RNA polymerase gene (rpoB)

被引：57

作者：

Kim, BJ

Hong, SK

Lee, KH

Yun, YJ

Kim, EC

Park, YG

Bai, GH

Kook, YH

机构：

[1] Seoul Natl Univ, Coll Med, Dept Microbiol, SNUMRC,Inst Endem DisChongno Gu, Seoul 110799, South Korea

[2] Seoul Natl Univ, Coll Med, SNUMRC, Inst Endem Dis,Canc Res Inst, Seoul 110799, South Korea

[3] Seoul Natl Univ, Coll Med, Dept Clin Pathol, Seoul 110799, South Korea

[4] Korean Natl TB Assoc, Korean Inst TB, Seoul 137140, South Korea

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2004年 / 42卷 / 03期

关键词：

D O I：

10.1128/JCM.42.3.1308-1312.2004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A novel duplex PCR method that can amplify the 235- and 136-bp rpoB DNAs of Mycobacterium tuberculosis complex and nontuberculous mycobacteria (NTM), respectively, with two different sets of primers was used to differentially identify 44 reference strains and 379 clinical isolates of mycobacteria in a single-step assay. Showing 100% sensitivity and specificity, the duplex PCR method could clearly differentiate M. tuberculosis complex and NTM strains. In addition, restriction fragment length polymorphism analysis and direct sequencing of the amplicon of NTM could be used to supplement species identification.

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页码：1308 / 1312

页数：5

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