Differentiation between Mycobacterium tuberculosis and Mycobacterium bovis by a multiplex-polymerase chain reaction

被引:16
作者
Herrera, EA [1 ]
Perez, O [1 ]
Segovia, M [1 ]
机构
[1] UNIV MURCIA,GEN HOSP,MICROBIOL SERV,DEPT GENET & MICROBIOL,MURCIA,SPAIN
来源
JOURNAL OF APPLIED BACTERIOLOGY | 1996年 / 80卷 / 06期
关键词
D O I
10.1111/j.1365-2672.1996.tb03263.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A multiplex-polymerase chain reaction (PCR) assay, based on one-step amplification and detection of three different mycobacterial genomic fragments, was designed for differentiation between Mycobacterium bovis and Mycobacterium tuberculosis. The oligonucleotide primers were chosen from the groEL gene, present in the genus Mycobacterium sp., from the IS6110 insertion sequence, present in Myco. tuberculosis complex and from the mtp40 gene, identified as a specific-species Myco. tuberculosis genomic fragment. This amplification method allowed the detection of two fragments of 576 and 317 base pairs in Myco. bovis and three fragments of 576, 396 and 317 base pairs in Myco. tuberculosis strains, including atypical strains of Myco. tuberculosis where the copy number of the IS6110 element is low. The multiplex-PCR assay described may be a very useful tool for the rapid and specific differentiation of these related mycobacteria and easy to use in medical and veterinary microbiology laboratories.
引用
收藏
页码:596 / 604
页数:9
相关论文
共 30 条
[1]   POLYMERASE CHAIN-REACTION FOR DETECTION OF MYCOBACTERIUM-TUBERCULOSIS IN SPUTUM [J].
ANDERSEN, AB ;
THYBO, S ;
GODFREYFAUSSETT, P ;
STOKER, NG .
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 1993, 12 (12) :922-927
[2]  
BRISSONNOEL A, 1989, LANCET, V2, P1069
[3]   IS6110 - CONSERVATION OF SEQUENCE IN THE MYCOBACTERIUM-TUBERCULOSIS COMPLEX AND ITS UTILIZATION IN DNA FINGERPRINTING [J].
CAVE, MD ;
EISENACH, KD ;
MCDERMOTT, PF ;
BATES, JH ;
CRAWFORD, JT .
MOLECULAR AND CELLULAR PROBES, 1991, 5 (01) :73-80
[4]   LARGE-SCALE USE OF POLYMERASE CHAIN-REACTION FOR DETECTION OF MYCOBACTERIUM-TUBERCULOSIS IN A ROUTINE MYCOBACTERIOLOGY LABORATORY [J].
CLARRIDGE, JE ;
SHAWAR, RM ;
SHINNICK, TM ;
PLIKAYTIS, BB .
JOURNAL OF CLINICAL MICROBIOLOGY, 1993, 31 (08) :2049-2056
[5]   BOVINE TUBERCULOSIS IN LATIN-AMERICA AND THE CARIBBEAN - CURRENT STATUS, CONTROL AND ERADICATION PROGRAMS [J].
DEKANTOR, IN ;
RITACCO, V .
VETERINARY MICROBIOLOGY, 1994, 40 (1-2) :5-14
[6]   AMPLIFICATION OF A SPECIES-SPECIFIC DNA FRAGMENT OF MYCOBACTERIUM-TUBERCULOSIS AND ITS POSSIBLE USE IN DIAGNOSIS [J].
DELPORTILLO, P ;
MURILLO, LA ;
PATARROYO, ME .
JOURNAL OF CLINICAL MICROBIOLOGY, 1991, 29 (10) :2163-2168
[7]   DIRECT DETECTION OF MYCOBACTERIUM-TUBERCULOSIS IN CLINICAL SPECIMENS BY DNA AMPLIFICATION [J].
DEWIT, D ;
STEYN, L ;
SHOEMAKER, S ;
SOGIN, M .
JOURNAL OF CLINICAL MICROBIOLOGY, 1990, 28 (11) :2437-2441
[8]   POLYMERASE CHAIN-REACTION AMPLIFICATION OF A REPETITIVE DNA-SEQUENCE SPECIFIC FOR MYCOBACTERIUM-TUBERCULOSIS [J].
EISENACH, KD ;
CAVE, MD ;
BATES, JH ;
CRAWFORD, JT .
JOURNAL OF INFECTIOUS DISEASES, 1990, 161 (05) :977-981
[9]   DETECTION OF MYCOBACTERIUM-TUBERCULOSIS IN SPUTUM SAMPLES USING A POLYMERASE CHAIN-REACTION [J].
EISENACH, KD ;
SIFFORD, MD ;
CAVE, MD ;
BATES, JH ;
CRAWFORD, JT .
AMERICAN REVIEW OF RESPIRATORY DISEASE, 1991, 144 (05) :1160-1163
[10]  
EISENSTEIN BI, 1990, NEW ENGL J MED, V322, P178