Differentiation between Mycobacterium tuberculosis and Mycobacterium bovis by a multiplex-polymerase chain reaction

被引:16
作者
Herrera, EA [1 ]
Perez, O [1 ]
Segovia, M [1 ]
机构
[1] UNIV MURCIA,GEN HOSP,MICROBIOL SERV,DEPT GENET & MICROBIOL,MURCIA,SPAIN
来源
JOURNAL OF APPLIED BACTERIOLOGY | 1996年 / 80卷 / 06期
关键词
D O I
10.1111/j.1365-2672.1996.tb03263.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A multiplex-polymerase chain reaction (PCR) assay, based on one-step amplification and detection of three different mycobacterial genomic fragments, was designed for differentiation between Mycobacterium bovis and Mycobacterium tuberculosis. The oligonucleotide primers were chosen from the groEL gene, present in the genus Mycobacterium sp., from the IS6110 insertion sequence, present in Myco. tuberculosis complex and from the mtp40 gene, identified as a specific-species Myco. tuberculosis genomic fragment. This amplification method allowed the detection of two fragments of 576 and 317 base pairs in Myco. bovis and three fragments of 576, 396 and 317 base pairs in Myco. tuberculosis strains, including atypical strains of Myco. tuberculosis where the copy number of the IS6110 element is low. The multiplex-PCR assay described may be a very useful tool for the rapid and specific differentiation of these related mycobacteria and easy to use in medical and veterinary microbiology laboratories.
引用
收藏
页码:596 / 604
页数:9
相关论文
共 30 条
[11]  
Kent P. T., 1985, PUBLIC HLTH MYCOBACT
[12]   INCIDENCE AND TREATMENT OF COMPLICATIONS OF BACILLUS-CALMETTE-GUERIN INTRAVESICAL THERAPY IN SUPERFICIAL BLADDER-CANCER [J].
LAMM, DL ;
VANDERMEIJDEN, APM ;
MORALES, A ;
BROSMAN, SA ;
CATALONA, WJ ;
HERR, HW ;
SOLOWAY, MS ;
STEG, A ;
DEBRUYNE, FMJ .
JOURNAL OF UROLOGY, 1992, 147 (03) :596-600
[13]   FALSE-POSITIVE RESULT OF MYCOBACTERIUM-TUBERCULOSIS COMPLEX DNA PROBE HYBRIDIZATION WITH A MYCOBACTERIUM-TERRAE ISOLATE [J].
MARTIN, C ;
LEVYFREBAULT, VV ;
CATTIER, B ;
LEGRAS, A ;
GOUDEAU, A .
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 1993, 12 (04) :309-310
[14]   RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM ANALYSIS USING IS6110 AS AN EPIDEMIOLOGIC MARKER IN TUBERCULOSIS [J].
OTAL, I ;
MARTIN, C ;
VINCENTLEVYFREBAULT, V ;
THIERRY, D ;
GICQUEL, B .
JOURNAL OF CLINICAL MICROBIOLOGY, 1991, 29 (06) :1252-1254
[15]   DETECTION AND IDENTIFICATION OF MYCOBACTERIUM-TUBERCULOSIS BY DNA AMPLIFICATION [J].
PAO, CC ;
YEN, TSB ;
YOU, JB ;
MAA, JS ;
FISS, EH ;
CHANG, CH .
JOURNAL OF CLINICAL MICROBIOLOGY, 1990, 28 (09) :1877-1880
[16]   ISOLATION, CHARACTERIZATION, AND MOLECULAR-CLONING OF A SPECIFIC MYCOBACTERIUM-TUBERCULOSIS ANTIGEN GENE - IDENTIFICATION OF A SPECIES-SPECIFIC SEQUENCE [J].
PARRA, CA ;
LONDONO, LP ;
DELPORTILLO, P ;
PATARROYO, ME .
INFECTION AND IMMUNITY, 1991, 59 (10) :3411-3417
[17]   DIFFERENTIATION OF MYCOBACTERIUM-TUBERCULOSIS AND MYCOBACTERIUM-BOVIS BCG BY A POLYMERASE CHAIN-REACTION ASSAY [J].
PLIKAYTIS, BB ;
EISENACH, KD ;
CRAWFORD, JT ;
SHINNICK, TM .
MOLECULAR AND CELLULAR PROBES, 1991, 5 (03) :215-219
[18]   A DNA PRIMER PROBE SYSTEM FOR THE RAPID AND SENSITIVE DETECTION OF MYCOBACTERIUM-TUBERCULOSIS-COMPLEX PATHOGENS [J].
RALPHS, NT ;
GARRETT, S ;
MORSE, R ;
COOKSON, JB ;
ANDREW, PW ;
BOULNOIS, GJ .
JOURNAL OF APPLIED BACTERIOLOGY, 1991, 70 (03) :221-226
[19]   PRIMER-DIRECTED ENZYMATIC AMPLIFICATION OF DNA WITH A THERMOSTABLE DNA-POLYMERASE [J].
SAIKI, RK ;
GELFAND, DH ;
STOFFEL, S ;
SCHARF, SJ ;
HIGUCHI, R ;
HORN, GT ;
MULLIS, KB ;
ERLICH, HA .
SCIENCE, 1988, 239 (4839) :487-491
[20]   THE 65-KILODALTON ANTIGEN OF MYCOBACTERIUM-TUBERCULOSIS [J].
SHINNICK, TM .
JOURNAL OF BACTERIOLOGY, 1987, 169 (03) :1080-1088