Sol-gel derived nanoporous cerium oxide film for application to cholesterol biosensor

被引：198

作者：

Ansari, Anees A. ^{[1
]}

Kaushik, A. ^{[1
]}

Solanki, P. R. ^{[1
]}

Malhotra, B. D. ^{[1
]}

机构：

[1] Natl Phys Lab, Biomol Elect & Conducting Polymer Res Grp, New Delhi 110012, India

来源：

ELECTROCHEMISTRY COMMUNICATIONS | 2008年 / 10卷 / 09期

关键词：

sol-gel; cerium oxide; nanoporous; cholesterol oxidase; electrochemical biosensor;

D O I：

10.1016/j.elecom.2008.06.003

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

Cholesterol oxidase (ChOx) has been immobilized onto sol-gel derived nano-structured cerium oxide (NS-CeO2) film deposited on indium-tin-oxide (ITO) coated glass substrate. Phase identification of sol-gel NS-CeO2 film carried out using X-ray diffraction (XRD) yields reflection peak at 29.4 degrees corresponding to (111) plane with oriented crystallite (34 nm) along c-axis normal to the substrate. Electrochemical studies reveal that NS-CeO2 provides electroactive surface for the loading of ChOx and enhances electron transfer rate in the ChOx/NS-CeO2/ITO bioelectrocle. The low value of Michaelis-Menten constant (K-m) obtained as 2.08 mM indicates enhanced ChOx affinity to cholesterol. The observed results show application of sol-gel derived NS-CeO2 for biosensing without any functionalization. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：1246 / 1249

页数：4

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