Zinc oxide nanoparticles-chitosan composite film for cholesterol biosensor

被引：233

作者：

Khan, Raju ^{[1
]}

Kaushik, Ajeet ^{[1
]}

Solanki, Pratima R. ^{[1
]}

Ansari, Anees A. ^{[1
]}

Pandey, Manoj K. ^{[1
]}

Malhotra, B. D. ^{[1
]}

机构：

[1] Natl Phys Lab, Biomol Elect & Conducting Polymer Res Grp, New Delhi 110012, India

来源：

ANALYTICA CHIMICA ACTA | 2008年 / 616卷 / 02期

关键词：

zinc oxide nanoparticles-chitosan film; cholesterol oxidase; cholesterol; biosensor;

D O I：

10.1016/j.aca.2008.04.010

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Zinc oxide nanoparticles (NanoZnO) uniformly dispersed in chitosan (CHIT) have been used to fabricate a hybrid nanocomposite film onto indium-tin-oxide (ITO) glass plate. Cholesterol oxidase (ChOx) has been immobilized onto this NanoZnO-CHIT composite film using physiosorption technique. Both NanoZnO-CHIT/ITO electrode and ChOx/NanoZnO-CHIT/ITO bioelectrode have been characterized using Fourier transform-infrared (FTIR), X-ray diffraction (XRD), cyclic voltammetry (CV), scanning electron microscopy (SEM) and electrochemical impedance spectroscopy (EIS) techniques, respectively. The ChOx/NanoZnO-CHIT/ITO bioelectrode exhibits linearity from 5 to 300 mg dl(-1) of cholesterol with detection limit as 5 mg dl(-1), sensitivity as 1.41 x 10(-4) A mg dl(-1) and the value of Michaelis-Menten constant (K-m) as 8.63 mg dl(-1). This cholesterol biosensor can be used to estimate cholesterol in serum samples. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：207 / 213

页数：7

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