Poly-(3-hexylthiophene) self-assembled monolayer based cholesterol biosensor using surface plasmon resonance technique

被引:69
作者
Arya, Sunil K.
Solanki, Pratima R.
Singh, S. P.
Kaneto, K.
Pandey, Manoj K.
Datta, Monika
Malhotra, Bansi D.
机构
[1] Natl Phys Lab, Biomol Electron & Conducting Polymer Res Grp, New Delhi 110012, India
[2] Kyushu Inst Technol, Dept Biol Funct & Engn, Grad Sch Life Sci & Syst Engn, Wakamitsu Ku, Kitakyushu, Fukuoka 8080196, Japan
[3] Univ Delhi, Dept Chem, Delhi 110007, India
基金
日本学术振兴会;
关键词
cholesterol; cholesterol oxidase (ChOx); surface plasmon resonance (SPR); poly-(3-hexylthiophene) (P3HT); electrochemical impedance; 1-fluoro-2-nitro-4-azidobenzene (FNAB);
D O I
10.1016/j.bios.2006.10.011
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Cholesterol oxidase (ChOx) has been covalently immobilized onto 1-fluoro-2-nitro-4-azidobenzene (FNAB) modified poly-(3-hexylthiophene) (P3HT) self-assembled monolayer (SAM) onto gold coated glass plates. These ChOx./FNAB/P3HT/Au bio-electrodes have been characterized using contact angle (CA) measurements, UV-vis spectroscopy, electrochemical impedance technique, cyclic voltammetric technique and atomic force microscopic (AFM) technique, respectively. The ChOx/FNAB/P3HT/Au bio-electrodes were utilized for the estimation of cholesterol concentration in standard solutions using surface plasmon resonance (SPR) technique. It is shown that this SPR biosensor has linearity from 50 to 500 mg/dl of cholesterol in solution with detection limit of 50 mg/dl, sensitivity of 1.04 m degrees/(mg dl), reusability of around 15 times and a shelf-life of about 10 weeks when stored at 4 degrees C. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码:2516 / 2524
页数:9
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