Vacuole acidification is required for trans-SNARE pairing, LMA1 release, and homotypic fusion

被引：92

作者：

Ungermann, C ^{[1
]}

Wickner, W ^{[1
]}

Xu, ZY ^{[1
]}

机构：

[1] Dartmouth Med Sch, Dept Biochem, Hanover, NH 03755 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1999年 / 96卷 / 20期

关键词：

D O I：

10.1073/pnas.96.20.11194

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Vacuole fusion occurs in three stages: priming, in which Sec18p mediates Sec17p release, LMA1 (low M-r activity 1) relocation, and cis-SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) complex disassembly; docking, mediated by Ypt7p and trans-SNARE association; and fusion of docked vacuoles. Ca2+ and calmodulin regulate late stages of the reaction. We now show that the vacuole proton gradient, generated by the vacuolar proton ATPase, is needed for trans-SNARE complex formation during docking and hence for the subsequent LMA1 release. Though neither the vacuolar Pmc1p Ca2+-ATPase nor the Vcx1p Ca2+/H+ exchanger are needed for the fusion reaction, they participate in Ca2+ and Delta mu(H)(+) homeostasis. Fusion itself does not require the maintenance of trans-SNARE pairs.

引用

页码：11194 / 11199

页数：6

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