共 73 条
Heritable Remodeling of Yeast Multicellularity by an Environmentally Responsive Prion
被引:129
作者:
Holmes, Daniel L.
[1
]
Lancaster, Alex K.
[2
]
Lindquist, Susan
[2
,3
,4
]
Halfmann, Randal
[1
]
机构:
[1] Univ Texas SW Med Ctr Dallas, Dept Biochem, Dallas, TX 75390 USA
[2] Whitehead Inst Biomed Res, Cambridge, MA 02142 USA
[3] MIT, Dept Biol, Cambridge, MA 02139 USA
[4] MIT, Howard Hughes Med Inst, Cambridge, MA 02139 USA
来源:
关键词:
SACCHAROMYCES-CEREVISIAE;
PSEUDOHYPHAL GROWTH;
DRUG-RESISTANCE;
GENE-EXPRESSION;
TRANSCRIPTION;
EVOLUTION;
PROTEIN;
HSP104;
PSI+;
FLOCCULATION;
D O I:
10.1016/j.cell.2013.02.026
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
Prion proteins undergo self-sustaining conformational conversions that heritably alter their activities. Many of these proteins operate at pivotal positions in determining how genotype is translated into phenotype. But the breadth of prion influences on biology and their evolutionary significance are just beginning to be explored. We report that a prion formed by the Mot3 transcription factor, [MOT3(+)], governs the acquisition of facultative multicellularity in the budding yeast Saccharomyces cerevisiae. The traits governed by [MOT3(+)] involved both gains and losses of Mot3 regulatory activity. [MOT3(+)]-dependent expression of FLO11, a major determinant of cell-cell adhesion, produced diverse lineage-specific multicellular phenotypes in response to nutrient deprivation. The prions themselves were induced by ethanol and eliminated by hypoxia-conditions that occur sequentially in the natural respiro-fermentative cycles of yeast populations. These data demonstrate that prions can act as environmentally responsive molecular determinants of multicellularity and contribute to the natural morphological diversity of budding yeast.
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页码:153 / 165
页数:13
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