FA-loaded lipid drug delivery systems: Preparation, characterization and biological studies

被引：76

作者：

Carbone, Claudia ^{[1
]}

Campisi, Agata ^{[2
]}

Musumeci, Teresa ^{[1
]}

Raciti, Giuseppina ^{[2
]}

Bonfanti, Roberta ^{[2
]}

Puglisi, Giovanni ^{[1
]}

机构：

[1] Univ Catania, Dept Drug Sci, Lab Drug Delivery Technol, I-95125 Catania, Italy

[2] Univ Catania, Dept Drug Sci, Biochem Sect, I-95125 Catania, Italy

来源：

EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES | 2014年 / 52卷

关键词：

Lipid nanoparticles; DSC; Ferulic acid; Glioblastoma; Cellular viability; Immunocytochemistry; FERULIC ACID; IN-VITRO; TISSUE TRANSGLUTAMINASE; NANOPARTICLES; FORMULATION; IDEBENONE; MATRICES; CULTURES; RELEASE;

D O I：

10.1016/j.ejps.2013.10.003

中图分类号：

R9 [药学];

学科分类号：

100702 [药剂学];

摘要：

The main purpose of this research was to prepare and to characterize ferulic acid-loaded nanostructured lipid carrier (FA-NLC) to evaluate the cytotoxic effect on human glioblastoma cancer U87MG cells. First of all, the influence of different materials on mean size and homogeneity of NLC prepared by a low energy organic solvent-free method was investigated. Technological characterization (encapsulation efficiency, mean particle size, homogeneity and in vitro relgase profile) was performed on the selected NLC in comparison to others lipid carriers, nanoemulsion and SLN. Furthermore, the thermal behavior of NLC and SLN was investigated using Differential Scanning Calorimetry (DSC) in order to evaluate their structure. Biological studies (MIT bioassay and caspase-3 cleavage) on the selected NLC showed no cytotoxic effects of the unloaded tested NLC. Besides, the effectiveness of FA-loaded NLC was higher compared to the free drug. Cells treated with FA or FA-loaded NLC showed a greater effect compared to idebenone (IDE) or IDE-loaded NLC, respectively. These results strongly support that FA-loaded NLC could be potentially used for the treatment of glioblastoma. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：12 / 20

页数：9

共 27 条

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