Utility of a set of microsatellite primers developed for the massasauga rattlesnake (Sistrurus catenatus) for population genetic studies of the timber rattlesnake (Crotalus horridus)

被引:7
作者
Anderson, Corey Devin [1 ]
机构
[1] Washington Univ, Dept Biol, St Louis, MO 63130 USA
来源
MOLECULAR ECOLOGY NOTES | 2006年 / 6卷 / 02期
关键词
amplification; cross-species; Crotalus; microsatellite DNA; rattlesnakes; Sistrurus;
D O I
10.1111/j.1471-8286.2006.01302.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
I tested six microsatellite DNA primer pairs developed for the massasauga rattlesnake (Sistrurus catenatus) on a sample population of the timber rattlesnake (Crotalus horridus). It had been speculated in a previous publication that cross-species amplification would not be worthwhile across the two rattlesnake genera. However, for this primer set (the only one currently published for the genus Sistrurus), successful amplification at each locus was accomplished for all loci with an annealing temperature of 57 degrees C and locus-specific buffer conditions. Each locus was polymorphic, with the number of alleles per locus ranging from two to 12. Significant heterozygote deficits were detected for three loci (Scu01, Scu05 and Scu07). For Scu01, all individuals were homozygous for the same allele except one female who was homozygous for a different allele. This same female was also homozygous for a rare allele at Scu07. When this female was removed from the data set, the number of observed heterozygotes at Scu01 and Scu07 did not differ significantly from random expectations. However, a large heterozygote deficit persisted at Scu05 (despite subsampling), suggesting that this locus may not be useful for population genetic studies of timber rattlesnakes. Despite some limitations, this set of primers may be a useful complement to those already developed for the genus Crotalus. Moreover, the results of this study seem to provide new justification for further studies of cross-species amplification of microsatellite loci across the two rattlesnake genera.
引用
收藏
页码:514 / 517
页数:4
相关论文
共 13 条
[1]  
Angers B, 1996, MOL ECOL, V5, P317, DOI 10.1111/j.1365-294X.1996.tb00322.x
[2]   Cross-species amplification of Crotalus horridus microsatellites and their application in phylogenetic analysis [J].
Bushar, LM ;
Maliga, M ;
Reinert, HK .
JOURNAL OF HERPETOLOGY, 2001, 35 (03) :532-537
[3]   Characterization of DNA microsatellite loci from a threatened snake:: The eastern massasauga rattlesnake (Sistrurus c. catenatus) and their use in population studies [J].
Gibbs, HL ;
Prior, K ;
Parent, C .
JOURNAL OF HEREDITY, 1998, 89 (02) :169-173
[4]   PCR primers for microsatellite loci in the tiger rattlesnake (Crotalus tigris, Viperidae) [J].
Goldberg, CS ;
Edwards, T ;
Kaplan, ME ;
Goode, M .
MOLECULAR ECOLOGY NOTES, 2003, 3 (04) :539-541
[5]   Isolation and characterization of microsatellite loci from a threatened rattlesnake (New Mexico Ridge-nosed Rattlesnake, Crotalus willardi obscurus) [J].
Holycross, AT ;
Douglas, ME ;
Higbee, JR ;
Bogden, RH .
MOLECULAR ECOLOGY NOTES, 2002, 2 (04) :537-539
[6]  
Jordan PW, 2002, MOL ECOL NOTES, V2, P78, DOI 10.1046/j.1471-8286.2002.00135.x
[7]   THE CONSERVATION OF DINUCLEOTIDE MICROSATELLITES AMONG MAMMALIAN GENOMES ALLOWS THE USE OF HETEROLOGOUS PCR PRIMER PAIRS IN CLOSELY RELATED SPECIES [J].
MOORE, SS ;
SARGEANT, LL ;
KING, TJ ;
MATTICK, JS ;
GEORGES, M ;
HETZEL, DJS .
GENOMICS, 1991, 10 (03) :654-660
[8]   A wide-range survey of cross-species microsatellite amplification in birds [J].
Primmer, CR ;
Moller, AP ;
Ellegren, H .
MOLECULAR ECOLOGY, 1996, 5 (03) :365-378
[9]   GENEPOP (VERSION-1.2) - POPULATION-GENETICS SOFTWARE FOR EXACT TESTS AND ECUMENICISM [J].
RAYMOND, M ;
ROUSSET, F .
JOURNAL OF HEREDITY, 1995, 86 (03) :248-249
[10]   Isolation and characterization of novel microsatellite markers from the Australian tiger snakes (Elapidae: Notechis) and amplification in the closely related genus Hoplocephalus [J].
Scott, IAW ;
Hayes, CM ;
Keogh, JS ;
Webb, JK .
MOLECULAR ECOLOGY NOTES, 2001, 1 (03) :117-119