Isolation and characterization of novel microsatellite markers from the Australian tiger snakes (Elapidae: Notechis) and amplification in the closely related genus Hoplocephalus

被引：28

作者：

Scott, IAW

Hayes, CM

Keogh, JS

Webb, JK

机构：

[1] Australian Natl Univ, Div Bot & Zool, Canberra, ACT 0200, Australia

[2] No Terr Univ, Key Ctr Trop Wildlife Management, Casuarina, NT 0909, Australia

来源：

MOLECULAR ECOLOGY NOTES | 2001年 / 1卷 / 03期

关键词：

elapid; Hoplocephalus; microsatellite; Notechis; snake;

D O I：

10.1046/j.1471-8278.2001.00037.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We provide details of seven microsatellite loci (out of 20 primer pairs designed) that exhibited the cleanest and strongest banding patterns for the Eastern tiger snake Notechis scutatus. These loci were used to screen 76 individuals from across the geographical range of N. scutatus and a further 14 individuals of the closely related and endangered broadheaded snake Hoplocephalus bungaroides. We observed large numbers of alleles per locus (14-52) and relatively high levels of heterozygosity (0.270-0.696) within the N. scutatus sample. These markers are also likely to be informative for work on H. bungaroides.

引用

页码：117 / 119

页数：3

共 7 条

[1]

Burns EL, 1999, MOL ECOL, V8, P520

[2]

Cogger H.C., 2000, Reptiles and amphibians of Australia

[3] Molecular phylogeny of viviparous Australian elapid snakes:: affinities of Echiopsis atriceps (Storr, 1980) and Drysdalia coronata (Schlegel, 1837), with description of a new genus [J].