Microinjection molded disposable microfluidic lab-on-a-chip for efficient detection of agglutination

被引:19
作者
Choi, Sung Hwan [1 ]
Kim, Dong Sung [2 ]
Kwon, Tai Hun [1 ]
机构
[1] Pohang Univ Sci & Technol, Dept Mech Engn, Pohang 790784, South Korea
[2] Chung Ang Univ, Sch Mech Engn, Seoul 156756, South Korea
来源
MICROSYSTEM TECHNOLOGIES-MICRO-AND NANOSYSTEMS-INFORMATION STORAGE AND PROCESSING SYSTEMS | 2009年 / 15卷 / 02期
关键词
SERPENTINE LAMINATING MICROMIXER; TOTAL ANALYSIS SYSTEMS; ADVECTION;
D O I
10.1007/s00542-008-0689-x
中图分类号
TM [电工技术]; TN [电子技术、通信技术];
学科分类号
0808 ; 0809 ;
摘要
Previous diagnosing methods based on agglutination have a limitation in view of emergency and point-of-care diagnoses due to the requirement of large scale equipments and much agglutination time. In this paper, we propose a low cost microfluidic lab-on-a-chip for more efficient detection of agglutination. In the present lab-on-a-chip, two inlet microwells, flow guiding microchannels, chaotic micromixer and reaction microwell are fully integrated. Mold inserts for the lab-on-a-chip were manufactured by UV photolithography and nickel electroplating process. The complete lab-on-a-chip was realized by the microinjection molding of cyclic olefin copolymer and the subsequent thermal bonding. The improved serpentine laminating micromixer, developed by our group, integrated in the lab-on-a-chip showed the high-level of chaotic mixing, thereby enabling us to get a reliable mixing of sample and reagent. The performance of the fabricated lab-on-a-chip was demonstrated by agglutination experiments with simulated bloods of 10 mu l and simulated sera of 10 mu l. The results of agglutination inside the reaction microwell were clearly read by means of the level of light transmission. The present microfluidic lab-on-a-chip could be widely applied to various clinical diagnostics based on agglutination tests.
引用
收藏
页码:309 / 316
页数:8
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