Mucosal immunization with purified ClpP could elicit protective efficacy against pneumococcal pneumonia and sepsis in mice

被引：12

作者：

Cao, Ju ^{[1
,2
]}

Chen, Tingmei ^{[1
]}

Li, Dairong ^{[1
]}

Wong, Chun K. ^{[2
]}

Chen, Dapeng ^{[1
,2
]}

Xu, Wenchun ^{[1
]}

Zhang, Xuemei ^{[1
]}

Lam, Christopher W. K. ^{[2
]}

Yin, Yibing ^{[1
]}

机构：

[1] Chongqing Med Univ, Minist Educ, Key Lab Diagnost Med Designated, Chongqing 400016, Peoples R China

[2] Chinese Univ Hong Kong, Prince Wales Hosp, Dept Chem Pathol, Shatin, Hong Kong, Peoples R China

来源：

MICROBES AND INFECTION | 2008年 / 10卷 / 14-15期

关键词：

ClpP; Vaccine; Mucosal immunization;

D O I：

10.1016/j.micinf.2008.09.007

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Caseinolytic protease (ClpP) has been found to be highly conserved among different strains of Streptococcus pneumoniae and intraperitoneal immunization with ClpP could elicit protection against invasive pneumococcal infections. In this study, mucosal immunization with ClpP antigen induced both systemic and mucosal antibodies, and in this way reduced lung colonization in an invasive pneumococcal pneumonia model and also protected mice against death in an intraperitoneal-sepsis model. Surface localization of ClpP was confirmed using flow cytometry analysis. Furthermore, characterization of human sera for anti-ClpP IgG antibody levels demonstrated that ClpP protein was immunogenic in healthy children and was expressed during disease based on the elevated antibody levels in infected individuals. Finally, we describe that in vitro functional anti-ClpP antibody could kill streptococcus pneumoniae by polymorphonuclear leukocytes in a complement-dependent assay. To our knowledge, this is the first study about the protective efficacy of mucosal immunization with ClpP as a promising pneumococcal protein antigen. (C) 2008 Elsevier Masson SAS. All rights reserved.

引用

收藏

页码：1536 / 1542

页数：7

相关论文

共 30 条

[1] Identification and characterization of a novel family of pneumococcal proteins that are protective against sepsis [J].

Adamou, JE ;

Heinrichs, JH ;

Erwin, AL ;

Walsh, W ;

Gayle, T ;

Dormitzer, M ;

Dagan, R ;

Brewah, YA ;

Barren, P ;

Lathigra, R ;

Langermann, S ;

Koenig, S ;

Johnson, S .

INFECTION AND IMMUNITY, 2001, 69 (02) :949-958

[2] Dendritic cells and the control of immunity [J].

Banchereau, J ;

Steinman, RM .

NATURE, 1998, 392 (6673) :245-252

[3] Antibody-independent, CD4+ T-Cell-Dependent protection against pneumococcal colonization elicited by intranasal immunization with purified pneumococcal proteins [J].

Basset, Alan ;

Thompson, Claudette M. ;

Hollingshead, Susan K. ;

Briles, David E. ;

Ades, Edwin W. ;

Lipsitch, Marc ;

Malley, Richard .

INFECTION AND IMMUNITY, 2007, 75 (11) :5460-5464

[4] The potential for using protein vaccines to protect against otitis media caused by Streptococcus pneumoniae [J].

Briles, DE ;

Hollingshead, SK ;

Nabors, GS ;

Paton, JC ;

Brooks-Walter, A .

VACCINE, 2000, 19 :S87-S95

[5] Enhanced protection against pneumococcal infection elicited by immunization with the combination of PspA, PspC, and ClpP [J].

Cao, Ju ;

Chen, Dapeng ;

Xu, Wenchun ;

Chen, Tingmei ;

Xu, Songxiao ;

Luo, Jingyong ;

Zhao, Qing ;

Liu, Beizhong ;

Wang, Dongsheng ;

Zhang, Xuemei ;

Shan, Youlan ;

Yin, Yibing .

VACCINE, 2007, 25 (27) :4996-5005

[6] Immunity to Streptococcus pneumoniae:: factors affecting production and efficacy [J].

Casal, J ;

Tarragó, D .

CURRENT OPINION IN INFECTIOUS DISEASES, 2003, 16 (03) :219-224

[7] Streptococcus pneumoniae pilus subunits protect mice against lethal challenge [J].

Gianfaldoni, Claudia ;

Censini, Stefano ;

Hilleringmann, Markus ;

Moschioni, Monica ;

Facciotti, Claudia ;

Pansegrau, Werner ;

Masignani, Vega ;

Covacci, Antonello ;

Rappuoli, Rino ;

Barocchi, Michele Anne ;

Ruggiero, Paolo .

INFECTION AND IMMUNITY, 2007, 75 (02) :1059-1062

[8] Discovery of a novel class of highly conserved vaccine antigens using genomic scale antigenic fingerprinting of pneumococcus with human antibodies [J].

Giefing, Carmen ;

Meinke, Andreas L. ;

Hanner, Markus ;

Henics, Tamas ;

Minh, Duc Bui ;

Gelbmann, Dieter ;

Lundberg, Urban ;

Senn, Beatrice M. ;

Schunn, Michael ;

Habel, Andre ;

Henriques-Normark, Birgitta ;

Oetqvist, Ake ;

Kalin, Mats ;

von Gabain, Alexander ;

Nagy, Eszter .

JOURNAL OF EXPERIMENTAL MEDICINE, 2008, 205 (01) :117-131

[9] PppA, a surface-exposed protein of Streptococcus pneumoniae, elicits cross-reactive antibodies that reduce colonization in a murine intranasal immunization and challenge model [J].

Green, BA ;

Zhang, Y ;

Masi, AW ;

Barniak, V ;

Wetherell, M ;

Smith, RP ;

Reddy, MS ;

Zhu, DZ .

INFECTION AND IMMUNITY, 2005, 73 (02) :981-989

[10] Illustration of pneumococcal polysaccharide capsule during adherence and invasion of epithelial cells [J].

Hammerschmidt, S ;

Wolff, S ;

Hocke, A ;

Rosseau, S ;

Müller, E ;

Rohde, M .

INFECTION AND IMMUNITY, 2005, 73 (08) :4653-4667