Nucleotide Sequence Requirements at the 5′ End of the Influenza A Virus M RNA Segment for Efficient Virus Replication

被引：65

作者：

Ozawa, Makoto ^{[2
,5
]}

Maeda, Junko ^{[4
]}

Iwatsuki-Horimoto, Kiyoko ^{[2
]}

Watanabe, Shinji ^{[5
]}

Goto, Hideo ^{[2
]}

Horimoto, Taisuke ^{[2
]}

Kawaoka, Yoshihiro ^{[1
,2
,3
,5
]}

机构：

[1] Univ Tokyo, Inst Med Sci, Int Res Ctr Infect Dis, Minato Ku, Tokyo 1088639, Japan

[2] Univ Tokyo, Dept Microbiol & Immunol, Div Virol, Minato Ku, Tokyo 1088639, Japan

[3] Japan Sci & Technol Agcy, ERATO Infect Induced Host Responses Project, Kawaguchi, Saitama 3320012, Japan

[4] Hokkaido Univ, Grad Sch Vet Med, Dept Prion Dis, Lab Prion Dis, Sapporo, Hokkaido 060818, Japan

[5] Univ Wisconsin, Sch Vet Med, Dept Pathobiol Sci, Madison, WI 53706 USA

来源：

JOURNAL OF VIROLOGY | 2009年 / 83卷 / 07期

基金：

日本科学技术振兴机构;

关键词：

PACKAGING SIGNALS; VIRAL-RNA; POLYADENYLATION SIGNAL; NONCODING REGIONS; POLYMERASE GENE; VIRIONS; PB2; CONSERVATION; GENERATION; PARTICLES;

D O I：

10.1128/JVI.02513-08

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The mechanism by which the influenza A virus genome is packaged into virions is not fully understood. The coding and noncoding regions necessary for packaging of the viral RNA segments, except for the M segment, have been identified. Here, we delineate the M segment regions by incorporating a reporter viral RNA into virions and by generating viruses possessing mutations in the regions. We found that, like the other segments, the M segment coding regions are essential for virion incorporation and that the nucleotide length rather than the nucleotide sequence of the 5' end of the coding region is important.

引用

页码：3384 / 3388

页数：5

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