Isolation of a new set of Aspergillus nidulans mutants defective in nuclear migration

被引：74

作者：

Xiang, X ^{[1
]}

Zuo, WQ ^{[1
]}

Efimov, VP ^{[1
]}

Morris, NR ^{[1
]}

机构：

[1] Univ Med & Dent New Jersey, Robert Wood Johnson Med Sch, Dept Pharmacol, Piscataway, NJ 08854 USA

来源：

CURRENT GENETICS | 1999年 / 35卷 / 06期

基金：

美国国家卫生研究院;

关键词：

A; nidulans; Arp1; cytoplasmic dynein; nuclear migration;

D O I：

10.1007/s002940050461

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

In the filamentous fungus Aspergillus nidulans, nuclear migration in the germ tube is mediated by cytoplasmic dynein. We have previously reported the characterization of four nud (nuclear distribution) genes, nudA, nudC, nudF and nudG, involved in this process. The nudA and nudG genes respectively encode for the heavy chain and the 8-kDa light chain of cytoplasmic dynein. In this work, we describe an improved method for the isolation of nud mutants that has led to the identification of at least ten additional nud loci. We have cloned one of the genes, nudK, and determined that it encodes the actin-related protein Arp I, which is a component of the dynactin complex. This provides the first evidence that dynactin is involved in nuclear migration in A. nidulans.

引用

页码：626 / 630

页数：5

共 43 条

[41] CHARACTERIZATION AND LOCALIZATION OF THE CYTOPLASMIC DYNEIN HEAVY-CHAIN IN ASPERGILLUS-NIDULANS [J].

XIANG, X ;

ROGHI, C ;

MORRIS, NR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (21) :9890-9894

[42] NUDF, A NUCLEAR MIGRATION GENE IN ASPERGILLUS-NIDULANS, IS SIMILAR TO THE HUMAN LIS-1 GENE REQUIRED FOR NEURONAL MIGRATION [J].

XIANG, X ;

OSMANI, AH ;

OSMANI, SA ;

XIN, M ;

MORRIS, NR .

MOLECULAR BIOLOGY OF THE CELL, 1995, 6 (03) :297-310

[43] CYTOPLASMIC DYNEIN IS INVOLVED IN NUCLEAR MIGRATION IN ASPERGILLUS-NIDULANS [J].

XIANG, X ;

BECKWITH, SM ;

MORRIS, NR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (06) :2100-2104

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