Applications of the green fluorescent protein in cell biology and biotechnology

被引：295

作者：

Misteli, T

Spector, DL

机构：

[1] Cold Spring Harbor Laboratory, Cold Spring Harbor, NY

来源：

NATURE BIOTECHNOLOGY | 1997年 / 15卷 / 10期

关键词：

fluorescence; protein localization; gene expression; screening;

D O I：

10.1038/nbt1097-961

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The recent emergence of an autofluorescent protein, the green fluorescent protein (GFP), has opened the door for the convenient use of intact living cells and organisms as experimental systems in fields ranging from cell biology to biomedicine. We present an overview of some of the major applications of GFP, namely its use in protein tagging and in monitoring gene expression as well as its potential in a variety of biological screens.

引用

页码：961 / 964

页数：4

共 45 条

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